Document Detail


Immunohistochemical analysis of apoptosis-related factors in lining epithelium of radicular cysts.
MedLine Citation:
PMID:  15610406     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Some studies suggest that apoptosis-related factors are involved in the inflammatory processes of marginal periodontal lesions. However, the role of apoptosis in periapical inflammatory lesions remains unclear. We investigated the possible role of apoptotic cell death in periapical inflammatory lesions by means of immunohistochemical analysis of apoptosis-related factors and use of a cell proliferation marker. METHODS: Paraffin-embedded sections of 19 radicular cysts (RCs), and five residual radicular cysts (RRCs) and control specimens of normal gingivae excised from seven cadavers were prepared and examined immunohistochemically with the use of monoclonal antibodies or polyclonal antisera against single-stranded DNA (ssDNA), p53, Bax, Bcl-2, caspase-3, Fas, Fas ligand (Fas-L), and Ki-67 antigen. RESULTS: Epithelium of gingiva, RCs, and RRCs showed expression of ssDNA in suprabasal and superficial epithelial cells and Ki-67 reactivity in basal and parabasal cells. Expression of Ki-67 and ssDNA in RCs and RRCs was slightly higher than that in gingiva. Both Ki-67 and ssDNA reactivity in RCs with intense inflammatory reactions or with thick lining epithelium were significantly stronger than those in RCs with less inflammatory reactions or with thin lining epithelium. Reactivity for p53 was noted sporadically in epithelium of gingiva, RCs, and RRCs, and p53 expression in RCs was significantly greater than that in gingiva. Ki-67 and ssDNA reactivity in RCs increased parallel to the degree of p53 expression. Bax and Bcl-2 were detected in some basal epithelial cells in RCs and RRCs as well as in gingiva. The ssDNA reactivity significantly increased parallel to Bax expression and slightly decreased parallel to Bcl-2 expression in lining epithelium of RCs. Caspase-3 was detected in superficial epithelial cells of both gingiva and lining epithelium of RCs and RRCs, and the distribution of these cells was compatible with the expression of ssDNA. Expression of Ki-67 and ssDNA in caspase-3-positive fields was significantly higher than that in caspase-3-negative fields in RCs. There was very limited expression of Fas and Fas-L in lining epithelium of RCs and RRCs as well as in gingiva. CONCLUSIONS: These data suggest that apoptosis-related factors are involved in the pathophysiologic activity of periapical inflammatory lesions. Such factors may be affected by the structure of lining epithelium and the degree of inflammatory change.
Authors:
T Suzuki; H Kumamoto; K Kunimori; K Ooya
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology     Volume:  34     ISSN:  0904-2512     ISO Abbreviation:  J. Oral Pathol. Med.     Publication Date:  2005 Jan 
Date Detail:
Created Date:  2004-12-21     Completed Date:  2005-04-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8911934     Medline TA:  J Oral Pathol Med     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  46-52     Citation Subset:  D; IM    
Affiliation:
Division of Maxillofacial Surgery, Department of Oral Medicine and Surgery, Tohoku University Graduate School of Dentistry, Sendai, Japan. takahiro@mail.tains.tohoku.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Adult
Antigens, CD95 / analysis
Apoptosis*
Caspases / analysis
Chi-Square Distribution
DNA, Single-Stranded / analysis
Epithelium / chemistry,  pathology
Fas Ligand Protein
Female
Humans
Immunohistochemistry
Ki-67 Antigen / analysis
Male
Membrane Glycoproteins / analysis
Middle Aged
Proto-Oncogene Proteins c-bcl-2 / analysis
Radicular Cyst / chemistry,  pathology*
Statistics, Nonparametric
Tumor Suppressor Protein p53 / analysis
Chemical
Reg. No./Substance:
0/Antigens, CD95; 0/DNA, Single-Stranded; 0/FASLG protein, human; 0/Fas Ligand Protein; 0/Ki-67 Antigen; 0/Membrane Glycoproteins; 0/Proto-Oncogene Proteins c-bcl-2; 0/Tumor Suppressor Protein p53; EC 3.4.22.-/Caspases

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