Document Detail

Immunohistochemical analysis of MMP-9, MMP-2 and TIMP-1, TIMP-2 expression in the central nervous system following infection with viral and bacterial meningitis.
MedLine Citation:
PMID:  19141395     Owner:  NLM     Status:  MEDLINE    
Matrix metalloproteinases (MMPs) are capable of degrading components of the basal lamina of cerebral vessels, thereby disrupting the blood-brain barrier and inducing leukocyte recruitment. This study provides comprehensive information regarding the cell specificity of matrix metalloproteinases (MMP-2, MMP-9) and their binding tissue inhibitors (TIMP-1, TIMP-2) in the central nervous system during viral and bacterial meningitis. Specifically, we evaluated the immunoreactivity of MMPs and TIMPs in various cell types in brain parenchyma and meninges obtained from autopsy tissues. We found that a higher proportion of endothelial cells were positive for MMP-9 during meningitis when compared to controls. In addition, the immunoreactivity of MMP-9 decreased and the immunoreactivity of TIMP-1 increased in astrocytes upon infection. Furthermore, the results of this study revealed that mononuclear cells were highly immunoreactive for TIMP-1, TIMP-2 and MMP-9 during viral meningitis and that the expression of TIMPs in polymorphonuclear cells was even higher during bacterial meningitis. Taken together the results of this study indicated that the central nervous system resident cells and inflammatory infiltrates contribute to MMPs activity and that the expression patterns vary between cell types and in response to viral and bacterial meningitis.
Artur Sulik; Lech Chyczewski
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Folia histochemica et cytobiologica / Polish Academy of Sciences, Polish Histochemical and Cytochemical Society     Volume:  46     ISSN:  1897-5631     ISO Abbreviation:  Folia Histochem. Cytobiol.     Publication Date:  2008  
Date Detail:
Created Date:  2009-01-14     Completed Date:  2009-02-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8502651     Medline TA:  Folia Histochem Cytobiol     Country:  Poland    
Other Details:
Languages:  eng     Pagination:  437-42     Citation Subset:  IM    
Department of Pediatric Infectious Diseases, Medical University of Białystok, Poland.
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MeSH Terms
Astrocytes / enzymology,  pathology
Central Nervous System / enzymology*,  microbiology,  pathology,  virology
Endothelial Cells / enzymology,  pathology
Matrix Metalloproteinase 2 / metabolism*
Matrix Metalloproteinase 9 / metabolism*
Meninges / enzymology,  pathology
Meningitis, Bacterial / enzymology*,  pathology
Meningitis, Viral / enzymology*,  pathology
Middle Aged
Staining and Labeling
Tissue Inhibitor of Metalloproteinase-1 / metabolism*
Tissue Inhibitor of Metalloproteinase-2 / metabolism*
Reg. No./Substance:
0/Tissue Inhibitor of Metalloproteinase-1; 127497-59-0/Tissue Inhibitor of Metalloproteinase-2; EC Metalloproteinase 2; EC Metalloproteinase 9

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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