Document Detail


Immunohistochemical analysis of MMP-9, MMP-2 and TIMP-1, TIMP-2 expression in the central nervous system following infection with viral and bacterial meningitis.
MedLine Citation:
PMID:  19141395     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Matrix metalloproteinases (MMPs) are capable of degrading components of the basal lamina of cerebral vessels, thereby disrupting the blood-brain barrier and inducing leukocyte recruitment. This study provides comprehensive information regarding the cell specificity of matrix metalloproteinases (MMP-2, MMP-9) and their binding tissue inhibitors (TIMP-1, TIMP-2) in the central nervous system during viral and bacterial meningitis. Specifically, we evaluated the immunoreactivity of MMPs and TIMPs in various cell types in brain parenchyma and meninges obtained from autopsy tissues. We found that a higher proportion of endothelial cells were positive for MMP-9 during meningitis when compared to controls. In addition, the immunoreactivity of MMP-9 decreased and the immunoreactivity of TIMP-1 increased in astrocytes upon infection. Furthermore, the results of this study revealed that mononuclear cells were highly immunoreactive for TIMP-1, TIMP-2 and MMP-9 during viral meningitis and that the expression of TIMPs in polymorphonuclear cells was even higher during bacterial meningitis. Taken together the results of this study indicated that the central nervous system resident cells and inflammatory infiltrates contribute to MMPs activity and that the expression patterns vary between cell types and in response to viral and bacterial meningitis.
Authors:
Artur Sulik; Lech Chyczewski
Related Documents :
7892225 - Radial glial cell transformation to astrocytes is bidirectional: regulation by a diffus...
19800825 - The growth hormone secretagogue hexarelin increases cell proliferation in neurogenic re...
20085655 - Adaptation of ns cells growth and differentiation to high-throughput screening-compatib...
10498695 - A glial cell arises from an additional division within the mechanosensory lineage durin...
8913355 - Evidence for distinction of the differentiation-inducing activities and cytostatic prop...
18221365 - Calcineurin is involved in the early activation of nmda-mediated cell death in mutant h...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Folia histochemica et cytobiologica / Polish Academy of Sciences, Polish Histochemical and Cytochemical Society     Volume:  46     ISSN:  1897-5631     ISO Abbreviation:  Folia Histochem. Cytobiol.     Publication Date:  2008  
Date Detail:
Created Date:  2009-01-14     Completed Date:  2009-02-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8502651     Medline TA:  Folia Histochem Cytobiol     Country:  Poland    
Other Details:
Languages:  eng     Pagination:  437-42     Citation Subset:  IM    
Affiliation:
Department of Pediatric Infectious Diseases, Medical University of Białystok, Poland. artsul@interia.pl
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Astrocytes / enzymology,  pathology
Central Nervous System / enzymology*,  microbiology,  pathology,  virology
Endothelial Cells / enzymology,  pathology
Female
Humans
Immunohistochemistry
Male
Matrix Metalloproteinase 2 / metabolism*
Matrix Metalloproteinase 9 / metabolism*
Meninges / enzymology,  pathology
Meningitis, Bacterial / enzymology*,  pathology
Meningitis, Viral / enzymology*,  pathology
Middle Aged
Staining and Labeling
Tissue Inhibitor of Metalloproteinase-1 / metabolism*
Tissue Inhibitor of Metalloproteinase-2 / metabolism*
Chemical
Reg. No./Substance:
0/Tissue Inhibitor of Metalloproteinase-1; 127497-59-0/Tissue Inhibitor of Metalloproteinase-2; EC 3.4.24.24/Matrix Metalloproteinase 2; EC 3.4.24.35/Matrix Metalloproteinase 9

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Adventitial lymphatics of internal carotid artery in healthy and atherosclerotic vessels.
Next Document:  Impairment of microcirculation in juvenile idiopathic arthritis - studies by nailfold videocapillaro...