Document Detail


Immunohistochemical analysis of Bcl-2 and Bax expression in relation to cell turnover and epithelial differentiation markers in the non-lactating human mammary gland epithelium.
MedLine Citation:
PMID:  10654069     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The expression of the apoptosis-related proteins Bcl-2 and Bax was investigated by immunohistochemistry in the normal non-lactating human mammary gland in relation to cell proliferation and apoptosis. In order to characterize individual Bax/Bcl-2-immunoreactive cells, the epithelial markers cytokeratin 14 and 19 and the macrophage marker CD 68 were used. Secretory-like differentiation of epithelial cells was characterized by histochemistry and lectin staining of surface glycoconjugates. Cell proliferation was exclusively found in glandular epithelial cells with broad contact to the ductular lumen, whereas nuclei with apoptosis-related DNA fragmentation were seen predominantly in basally located glandular epithelial cells and in myoepithelial cells. Weak immunoreactivity for Bcl-2 and Bax was present throughout all epithelia, suggesting a balance between pro- and antiapoptotic effects in the majority of epithelial cells. However, specific cells showed a strong staining for Bax or Bcl-2. The strongly Bcl-2-immunoreactive epithelial cells were not identical with proliferating cells, but they resembled them in configuration and in the luminal intraepithelial position. In contrast, the strongly Bax-positive epithelial cells had no or only a narrow contact to the ductular lumen. The different patterns of Bax/Bcl-2 immunoreactivity in specific glandular epithelial cells suggest that there are also different grades of susceptibility towards apoptotic stimuli in individual glandular epithelial cells. We conclude that specific Bax/Bcl-2 expression patterns could reflect particular cell differentiation states, and that the strongly Bcl-2-positive cells in part could represent epithelial stem cells.
Authors:
F Feuerhake; W Sigg; E A Höfter; T Dimpfl; U Welsch
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell and tissue research     Volume:  299     ISSN:  0302-766X     ISO Abbreviation:  Cell Tissue Res.     Publication Date:  2000 Jan 
Date Detail:
Created Date:  2000-02-15     Completed Date:  2000-02-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0417625     Medline TA:  Cell Tissue Res     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  47-58     Citation Subset:  IM    
Affiliation:
Neurozentrum, Abteilung Neuropathologie, Freiburg, Germany. feuerhak@nzl1.ukl.uni-freiburg.de
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MeSH Terms
Descriptor/Qualifier:
Adult
Agglutinins
Antigens, CD / analysis*
Antigens, Differentiation, Myelomonocytic / analysis*
Apoptosis*
Biological Markers / analysis
Breast / cytology*
Cell Differentiation
Cell Division
Epithelial Cells / cytology*,  physiology*
Female
Humans
Immunohistochemistry
In Situ Nick-End Labeling
Keratin-14
Keratins / analysis*
Mammaplasty
Menstrual Cycle
Middle Aged
Proto-Oncogene Proteins / analysis*
Proto-Oncogene Proteins c-bcl-2 / analysis*
bcl-2-Associated X Protein
Chemical
Reg. No./Substance:
0/Agglutinins; 0/Antigens, CD; 0/Antigens, Differentiation, Myelomonocytic; 0/BAX protein, human; 0/Biological Markers; 0/CD68 antigen, human; 0/KRT14 protein, human; 0/Keratin-14; 0/Proto-Oncogene Proteins; 0/Proto-Oncogene Proteins c-bcl-2; 0/bcl-2-Associated X Protein; 68238-35-7/Keratins

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