| Immunohistochemical analyses on albumin and immunoglobulin in acute hypertensive mouse kidneys by "in vivo cryotechnique". | |
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MedLine Citation:
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PMID: 15944930 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The purpose of this study is to visualize topographical changes of serum proteins, albumin and immunoglobulin, passing through mouse glomerular capillary loops and their reabsorption in renal proximal tubules by immunohistochemistry in combination with our "in vivo cryotechnique". The "in vivo cryotechnique" was performed on left mouse kidneys under normotensive, experimentally acute hypertensive and heart-arrest conditions. The cryofixed tissues by the technique were routinely processed for freeze-substitution. Serial deparaffinized sections were stained with hematoxylin-eosine and immunostained with anti-mouse albumin, immunoglobulin G (IgG), kappa or lambda light chain and IgG1 heavy chain antibodies. Under the normotensive and heart-arrest conditions, albumin and IgG were clearly immunolocalized in blood vessels and slightly in apical cytoplasmic parts of some proximal tubules. Under the acute hypertensive condition, the albumin and kappa or lambda light chains, but not IgG1 heavy chain, were strongly immunolocalized in the apical cytoplasm of almost all proximal tubules. This study is the first in vivo visualization for glomerular passage of serum proteins and their transtubular absorption. Thus, the "in vivo cryotechnique" with freeze-substitution can be used for clarifying not only the functional morphology of living animal cells, but also in situ immunohistochemical localization of their components. |
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Authors:
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Z Li; N Terada; N Ohno; S Ohno |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Histology and histopathology Volume: 20 ISSN: 0213-3911 ISO Abbreviation: Histol. Histopathol. Publication Date: 2005 Jul |
Date Detail:
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Created Date: 2005-06-09 Completed Date: 2005-08-29 Revised Date: 2005-11-17 |
Medline Journal Info:
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Nlm Unique ID: 8609357 Medline TA: Histol Histopathol Country: Spain |
Other Details:
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Languages: eng Pagination: 807-16 Citation Subset: IM |
Affiliation:
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Department of Anatomy, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, 1110 Shimokato, Tamaho, Yamanashi 409-3898, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acute Disease Albumins / analysis* Animals Freeze Substitution / instrumentation, methods Hypertension, Renal / metabolism, pathology* Immunoglobulin G / analysis Immunoglobulin Heavy Chains / analysis Immunoglobulin Light Chains / analysis Immunoglobulins / analysis* Immunohistochemistry Kidney Tubules, Proximal / chemistry, pathology Mice Mice, Inbred C57BL |
| Chemical | |
Reg. No./Substance:
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0/Albumins; 0/Immunoglobulin G; 0/Immunoglobulin Heavy Chains; 0/Immunoglobulin Light Chains; 0/Immunoglobulins |
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