| An immunohistochemical study on the distribution and frequency of T regulatory cells in pancreatic islets of NOD mice during various stages of spontaneous and cyclophosphamide-accelerated diabetes. | |
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MedLine Citation:
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PMID: 20442677 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVES: To determine if there is an abrupt change in the frequency of intraislet T regulatory (Treg) cells in female nonobese diabetic (NOD) mice preceding and following spontaneous diabetes and during cyclophosphamide-accelerated disease. METHODS: The frequency of intraislet CD4-positive Treg cells was analyzed between days 21 and 250, at onset, and at 1, 2, and 3 weeks of diabetes by dual-label immunohistochemistry. Tissues were also analyzed between days 0 and 14 after injection of cyclophosphamide or diluent. RESULTS: In the spontaneous group, intraislet Treg cells were first observed on day 30 in CD4 T cells and increased from day 45. There was no statistical difference in the frequency of Treg cells in nondiabetic NOD mice on days 45, 60, and 90. A sustained frequency at and after 1, 2, and 3 weeks of diabetes was also observed. In the cyclophosphamide group, there was a sharp decline in the frequency of Treg cells on day 4, which remained lower on day 7 but increased by days 11 and 14. CONCLUSIONS: During spontaneous diabetes and after onset, the frequency of intraislet CD4-positive Treg cells remains unchanged. They may possess diminished immunoregulatory function and thus unable to counteract the increasing tempo of immune-mediated beta-cell destruction. |
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Authors:
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Sarbjot Kaur; Wan Ling Tan; Camelia Soo; Carlos Chun Ho Cheung; Joanna Stewart; Shiva Reddy |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Pancreas Volume: 39 ISSN: 1536-4828 ISO Abbreviation: Pancreas Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-09-23 Completed Date: 2011-01-13 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8608542 Medline TA: Pancreas Country: United States |
Other Details:
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Languages: eng Pagination: 1024-33 Citation Subset: IM |
Affiliation:
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School of Biological Sciences, The University of Auckland, Auckland, New Zealand. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cyclophosphamide / toxicity* Diabetes Mellitus / chemically induced, immunology* Female Immunohistochemistry Islets of Langerhans / pathology* Mice Mice, Inbred NOD T-Lymphocytes, Regulatory / physiology* |
| Chemical | |
Reg. No./Substance:
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50-18-0/Cyclophosphamide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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