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Immunohistochemical localization of oestrogen receptor alpha in the various cell categories of chick embryo ovary.
MedLine Citation:
PMID:  20887390     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
The immunohistochemical (IHC) localization of oestrogen receptor alpha (ERα) was studied in the developing left ovary of 14.5-day-old chick embryos. The study was focused in particular on distinguishing in cortex and medulla the different cell categories that proved positive to the reaction, in order to gain further understanding of gonadal cell interactions during ovarian development. Immunostained cells were observed in both the cortex and medulla, but the reactivity for ERα was discontinuous, probably due to variable cell requirements. In the cortex, positivity was observed in cells of the ovarian surface epithelium, in germ cells and in prefollicular cells. In the medulla, positivity was found in the following cell categories: interstitial cells, poorly differentiated somatic cord cells, including those delimiting lacunae, germ cells and their accompanying cells of epithelial origin. Furthermore, the IHC results showed that the intracellular localization of the antigen was cytoplasmic, nuclear, or both. The significance of ERα presence and intracellular localization was discussed in relation and as supplementary to previous research by various Authors. In particular, as regards the unusual cytoplasmic immunoreactivity, a gradual shift of ERα localization from cytoplasmic to nuclear during the embryonic period is suggested.
Authors:
A Civinini; C Chimenti; V P Gallo
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-09-30
Journal Detail:
Title:  Anatomia, histologia, embryologia     Volume:  39     ISSN:  1439-0264     ISO Abbreviation:  Anat Histol Embryol     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-11-22     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7704218     Medline TA:  Anat Histol Embryol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  546-54     Citation Subset:  IM    
Copyright Information:
© 2010 Blackwell Verlag GmbH.
Affiliation:
Department of Biology and Biotechnologies, Charles Darwin University of Rome, Viale dell'Università 32, 00185 Rome, Italy.
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