Document Detail

Immunohistochemical demonstration of the mineralocorticoid receptor, 11beta-hydroxysteroid dehydrogenase-1 and -2, and hexose-6-phosphate dehydrogenase in rat ovary.
MedLine Citation:
PMID:  19255253     Owner:  NLM     Status:  MEDLINE    
An IHC survey using several monoclonal antibodies against different portions of the rat mineralocorticoid receptor (MR) molecule demonstrated significant specific MR immunoreactivity in the ovary, prompting further study of the localization of MR and of determinants of extrinsic MR ligand specificity, 11beta-hydroxysteroid dehydrogenase (11beta-HSD) types 1 and 2, and hexose-6-phosphate dehydrogenase (H6PDH). MR expression (real-time RT-PCR and Western blot) did not differ significantly in whole rat ovaries at early diestrus, late diestrus, estrus, and a few hours after ovulation. MR immunostaining was most intense in corporal lutea cells, light to moderate in oocytes and granulosa cells, and least intense in theca cells. Light immunoreactivity for 11beta-HSD2 occurred in most cells, with some mural granulosa cells of mature follicles staining more strongly. The distribution of immunoreactivity for 11beta-HSD1 and H6PDH required to generate NADPH, the cofactor required for reductase activity of 11beta-HSD1, was similar, with the most-intense staining in the cytoplasm of corporal lutea and theca cells and light or no staining in the granulosa and oocytes. MR function in the ovary is as yet unclear, but distinct patterns of distribution of 11beta-HSD1 and -2 and H6PDH suggest that the ligand for MR activation in different cells of the ovary may be differentially regulated.
Elise P Gomez-Sanchez; Miriam T Gomez-Sanchez; Angela F de Rodriguez; Damian G Romero; Mary P Warden; Maria W Plonczynski; Celso E Gomez-Sanchez
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2009-03-02
Journal Detail:
Title:  The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society     Volume:  57     ISSN:  0022-1554     ISO Abbreviation:  J. Histochem. Cytochem.     Publication Date:  2009 Jul 
Date Detail:
Created Date:  2009-06-22     Completed Date:  2009-07-28     Revised Date:  2010-09-22    
Medline Journal Info:
Nlm Unique ID:  9815334     Medline TA:  J Histochem Cytochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  633-41     Citation Subset:  IM    
Research Service, G.V. (Sonny) Montgomery Veterans Affairs Medical Center and Division of Endocrinology, Department of Medicine, The University of Mississippi Medical Center, Jackson, Mississippi, USA.
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MeSH Terms
11-beta-Hydroxysteroid Dehydrogenase Type 1 / metabolism*
11-beta-Hydroxysteroid Dehydrogenase Type 2 / metabolism*
Antibodies, Monoclonal
Carbohydrate Dehydrogenases / metabolism*
Estrous Cycle
Ovary / metabolism*
Rats, Sprague-Dawley
Receptors, Mineralocorticoid / immunology,  metabolism*
Grant Support
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Receptors, Mineralocorticoid; EC 1.1.-/Carbohydrate Dehydrogenases; EC 1.1.1.-/galactose-6-phosphate dehydrogenase; EC Dehydrogenase Type 1; EC Dehydrogenase Type 2

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