Document Detail


Immunogold detection of types I and II chondrocyte collagen fibrils: an in situ atomic force microscopic investigation.
MedLine Citation:
PMID:  16586488     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Chondrocyte tissue engineering is a major challenge in the field of cartilage repair. The phenotype of chondrocytes consists of cartilage specific proteoglycan and type II collagen. During serial passages, chondrocytes dedifferentiate into cells, presenting a fibroblast-like phenotype consisting predominately of type I collagen synthesis. Observation of native collagen fibers could be visualized by atomic force microscope. Here, we developed an original and useful atomic force microscopy-based immunogold technique allowing biochemical distinction between types I and II collagen fibers. Imaging of 40-nm gold particles staining collagen fibers was performed in tapping mode. Rat 1 fibroblasts and human chondrosarcoma cells were used as positive models for types I and II collagen, respectively. As demonstrated by our data, primary rat chondrocytes adhering for 48 h on a glass substrate synthesize type II collagen native fibers. This technique allows analyses of local areas of the extracellular matrix of fixed cells, providing complementary data about cartilage phenotype. This simple approach could be of major interest for the biologist community in routine laboratory investigations, to localize in situ, macromolecules of the extracellular matrix.
Authors:
Youri Arntz; Laurent Jourdainne; Géraldine Greiner-Wacker; Simon Rinckenbach; Joëlle Ogier; Jean-Claude Voegel; Philippe Lavalle; Dominique Vautier
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Microscopy research and technique     Volume:  69     ISSN:  1059-910X     ISO Abbreviation:  Microsc. Res. Tech.     Publication Date:  2006 Apr 
Date Detail:
Created Date:  2006-04-10     Completed Date:  2006-05-31     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9203012     Medline TA:  Microsc Res Tech     Country:  United States    
Other Details:
Languages:  eng     Pagination:  283-90     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2006 Wiley-Liss, Inc.
Affiliation:
INSERM, U 595, Strasbourg, F-67085, France.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cells, Cultured
Chondrocytes / metabolism*,  ultrastructure
Collagen Type I / metabolism*
Collagen Type II / metabolism*
Fibrillar Collagens / metabolism*
Fibroblasts / metabolism*,  ultrastructure
Humans
Immunohistochemistry
Microscopy, Atomic Force
Rats
Chemical
Reg. No./Substance:
0/Collagen Type I; 0/Collagen Type II; 0/Fibrillar Collagens

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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