Document Detail

Immunoglobulins to group A streptococcal surface molecules decrease adherence to and invasion of human pharyngeal cells.
MedLine Citation:
PMID:  9488384     Owner:  NLM     Status:  MEDLINE    
The M protein is one of the most important virulence factors of group A streptococci (Streptococcus pyogenes) and may play an important role in the first steps of streptococcal infection. Since acute pharyngitis is a frequently occurring infectious disease caused by these bacteria, we wished to know whether antibodies to the M protein or other surface components inhibit adherence and internalization of streptococci to pharyngeal cells. We investigated the role of whole human secretory immunoglobulin A (sIgA), M6 protein-specific sIgA, and M6 protein-specific serum IgG in the inhibition of streptococcal adherence and internalization to cultured human pharyngeal cells. S. pyogenes D471, which produces a type 6 M protein (M+), and its isogenic M-negative (M-) derivative JRS75 were tested. Purified whole sIgA, M protein-specific sIgA, and sIgA preabsorbed with M protein were able to decrease significantly the adherence of streptococci to pharyngeal cells. Purified IgG against the M6 protein did not diminish the attachment of streptococci to the pharyngeal cells but did reduce internalization. Thus, our data suggest that secretory IgA may play a key role in preventing streptococcal infection at mucosal surfaces by blocking adherence while affinity-purified anti-M protein-specific IgG blocks epitopes responsible for invasion.
U Fluckiger; K F Jones; V A Fischetti
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Infection and immunity     Volume:  66     ISSN:  0019-9567     ISO Abbreviation:  Infect. Immun.     Publication Date:  1998 Mar 
Date Detail:
Created Date:  1998-03-12     Completed Date:  1998-03-12     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  974-9     Citation Subset:  IM    
Laboratory of Bacterial Pathogenesis and Immunology, The Rockefeller University, New York, New York 10021, USA.
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MeSH Terms
Antibodies, Bacterial / physiology*
Antigens, Bacterial*
Bacterial Adhesion*
Bacterial Outer Membrane Proteins*
Bacterial Proteins / immunology*
Carrier Proteins*
Colchicine / pharmacology
Cytochalasin D / pharmacology
Immunoglobulin A, Secretory / physiology*
Immunoglobulin G / physiology*
Pharynx / microbiology*
Streptococcus pyogenes / immunology*,  physiology
Tumor Cells, Cultured
Grant Support
Reg. No./Substance:
0/Antibodies, Bacterial; 0/Antigens, Bacterial; 0/Bacterial Outer Membrane Proteins; 0/Bacterial Proteins; 0/Carrier Proteins; 0/Immunoglobulin A, Secretory; 0/Immunoglobulin G; 0/streptococcal M protein; 22144-77-0/Cytochalasin D; 64-86-8/Colchicine

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