Document Detail

Immunoglobulin turnover in B lymphocyte subpopulations.
MedLine Citation:
PMID:  1086238     Owner:  NLM     Status:  MEDLINE    
"In vitro" turnover of leucine-labeled and of radioiodinated IgM has been studied with cells from various lymphoid organs of nude mice, i.e. lymph nodes, thoracic duct, spleen and bone marrow, as well as with subpopulations of B cells from spleen and bone marrow separated by free flow electrophoresis. Three types of IgM-producing lymphocytes could be distinguished by their turnover rates of IgM, by the size of the released IgM and by the capacity of the IgM molecules to be labeled by the lactoperoxidase-catalyzed radioiodination reaction and/or by incorporation of radioactive leucine. Type I cells release 7-8 S IgM rapidly (t1/2 = 1-3h); the released IGM is leucine-labeled and radioiodinated. Type II cells release 7-8 S IgM slowly (t1/2 =10-30); the released IgM is leucine labeled and radioiodinated. Type III cells release 19 S IgM rapidly (t1/2 =2-4 h); the released IgM is leucine labeled, but not radioiodinated. Lymph nodes and thoracic duct contain predominantly type II cells, bone marrow contains type I and II cells, spleen contains type I,II and III cells. It is suggested that type III cells are Ig-secreting plaque-forming plasma cells, type II cells are small, resting "memory" B cells, and type I cells may be newly formed antigen-inexperienced B cells.
F Melchers; R E Cone; H von Boehmer; J Sprent
Related Documents :
2963858 - Expression of a 33-kda antigen tm 1 on lymphocyte surface after modulation of cell surf...
2872228 - Selective partial igm deficiency: functional assessment of t and b lymphocytes in vitro.
8681608 - Leukaemic b-cell maturation: studies on immunoglobulin secretion mediated by two signal...
7589078 - Cd27/cd70 interaction directly drives b cell igg and igm synthesis.
19733258 - Lysophosphatidic acid mediates migration of human mesenchymal stem cells stimulated by ...
9188038 - Terminal dutp nick end labeling (tunel) positive cells in the different regions of the ...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  European journal of immunology     Volume:  5     ISSN:  0014-2980     ISO Abbreviation:  Eur. J. Immunol.     Publication Date:  1976 Jun 
Date Detail:
Created Date:  1977-01-03     Completed Date:  1977-01-03     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  1273201     Medline TA:  Eur J Immunol     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  382-8     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Antibody-Producing Cells
B-Lymphocytes / cytology,  immunology*,  metabolism
Bone Marrow / immunology,  physiology
Bone Marrow Cells
Cell Differentiation
Cell Separation
Immunoglobulin M / metabolism*
Lymph Nodes / immunology
Mice, Nude
Molecular Weight
Receptors, Antigen, B-Cell / metabolism*
Spleen / immunology,  physiology
Reg. No./Substance:
0/Immunoglobulin M; 0/Receptors, Antigen, B-Cell

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Recruitment of effector lymphocytes by initiator lymphocytes: role of a trypsin-sensitive membrane c...
Next Document:  5-Hydroxytryptophan effect on the development of the immune response: IgM and IgG antibodies and ros...