Document Detail


Immunoglobulin turnover in B lymphocyte subpopulations.
MedLine Citation:
PMID:  1086238     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
"In vitro" turnover of leucine-labeled and of radioiodinated IgM has been studied with cells from various lymphoid organs of nude mice, i.e. lymph nodes, thoracic duct, spleen and bone marrow, as well as with subpopulations of B cells from spleen and bone marrow separated by free flow electrophoresis. Three types of IgM-producing lymphocytes could be distinguished by their turnover rates of IgM, by the size of the released IgM and by the capacity of the IgM molecules to be labeled by the lactoperoxidase-catalyzed radioiodination reaction and/or by incorporation of radioactive leucine. Type I cells release 7-8 S IgM rapidly (t1/2 = 1-3h); the released IGM is leucine-labeled and radioiodinated. Type II cells release 7-8 S IgM slowly (t1/2 =10-30); the released IgM is leucine labeled and radioiodinated. Type III cells release 19 S IgM rapidly (t1/2 =2-4 h); the released IgM is leucine labeled, but not radioiodinated. Lymph nodes and thoracic duct contain predominantly type II cells, bone marrow contains type I and II cells, spleen contains type I,II and III cells. It is suggested that type III cells are Ig-secreting plaque-forming plasma cells, type II cells are small, resting "memory" B cells, and type I cells may be newly formed antigen-inexperienced B cells.
Authors:
F Melchers; R E Cone; H von Boehmer; J Sprent
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  European journal of immunology     Volume:  5     ISSN:  0014-2980     ISO Abbreviation:  Eur. J. Immunol.     Publication Date:  1976 Jun 
Date Detail:
Created Date:  1977-01-03     Completed Date:  1977-01-03     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  1273201     Medline TA:  Eur J Immunol     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  382-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibody-Producing Cells
B-Lymphocytes / cytology,  immunology*,  metabolism
Bone Marrow / immunology,  physiology
Bone Marrow Cells
Cell Differentiation
Cell Separation
Immunoglobulin M / metabolism*
Lymph Nodes / immunology
Mice
Mice, Nude
Molecular Weight
Receptors, Antigen, B-Cell / metabolism*
Spleen / immunology,  physiology
Chemical
Reg. No./Substance:
0/Immunoglobulin M; 0/Receptors, Antigen, B-Cell

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