Document Detail

Immunoglobulin receptor signaling depends on the carboxyl terminus but not the heavy-chain class.
MedLine Citation:
PMID:  2494658     Owner:  NLM     Status:  MEDLINE    
To examine the isotypic and structural requirements involved in signaling through the immunoglobulin (Ig) receptor on B lymphocytes, we established a panel of T15 idiotype-positive transfectants that expressed wild-type IgM, wild-type IgD, or hybrid IgM molecules. Growth inhibition of the transfected lymphoma cells in response to anti-idiotype antibodies was used to measure signaling. Hybrid IgM molecules were constructed so that the membrane-spanning region of the mu heavy chain was replaced by that of delta, gamma 2b, or alpha heavy chains or that of the I-Ab class II (Ia) alpha chain. All transfectants that expressed IgM or hybrid IgM molecules with membrane-spanning regions from another Ig isotype underwent signaling in response to anti-idiotype antibodies, whereas the IgM-Ia hybrid transfectants did not. Transfectants that expressed wild-type IgD molecules also underwent signaling, although this response was particularly sensitive to serum concentrations. These results imply that signaling occurs in a similar manner through heavy-chain receptors of any isotype and suggest that conserved amino acid sequences in the transmembrane regions are important in this process.
C F Webb; C Nakai; P W Tucker
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  86     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1989 Mar 
Date Detail:
Created Date:  1989-04-28     Completed Date:  1989-04-28     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1977-81     Citation Subset:  IM    
Department of Microbiology, University of Texas Southwestern Medical Center, Dallas 75235.
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MeSH Terms
Amino Acid Sequence
B-Lymphocytes / immunology*
Cell Division
Cloning, Molecular
Fluorescent Antibody Technique
Histocompatibility Antigens Class II / genetics,  immunology
Immunoglobulin D / genetics,  immunology
Immunoglobulin Heavy Chains / genetics,  immunology*
Immunoglobulin Idiotypes / immunology
Immunoglobulin Isotypes / genetics,  immunology
Immunoglobulin M / genetics,  immunology
Molecular Sequence Data
Receptors, Immunologic / immunology*
Signal Transduction*
Structure-Activity Relationship
Tumor Cells, Cultured
Reg. No./Substance:
0/Histocompatibility Antigens Class II; 0/Immunoglobulin D; 0/Immunoglobulin Heavy Chains; 0/Immunoglobulin Idiotypes; 0/Immunoglobulin Isotypes; 0/Immunoglobulin M; 0/Receptors, Immunologic

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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