Document Detail

Immunoglobulin prolongs survival of pig kidneys perfused ex vivo with human blood.
MedLine Citation:
PMID:  9652825     Owner:  NLM     Status:  MEDLINE    
Intravenous immunoglobulin (IVIG) (Octagam), was used to determine the effect on hyperacute rejection in an ex vivo xenograft model. Six pig kidneys were perfused with IVIG and fresh human AB blood, and six control pig kidneys were simultaneously perfused with albumin and blood from the same donation. The survival of the IVIG-perfused xenografts (median, 6.5 h) was significantly (P = 0.03) longer than the albumin-perfused xenografts (median, 3.5 h). Complement was activated in both groups. The administration of IVIG to the perfused blood resulted in immediate and significantly higher complement activation in the fluid phase as compared with the albumin group. At rejection the fluid phase complement activation was higher in the IVIG group than in the albumin group for C1rs/C1inh complexes, C4bc, Bb and TCC. At the time of rejection both the albumin and the IVIG group demonstrated interstitial tubular haemorrhage, vasculitis or necrosis of glomerular capillaries and glomerular microthrombi. IgM, C1q, C3c, C4 and fibrin were located in arteries and glomeruli and IgG in the interstitium in both groups at rejection. The fluid phase findings are consistent with a modulatory effect of IVIG on complement activation by deviating the classical pathway activation towards the fluid phase. The prolonged survival of the IVIG-perfused kidneys suggests that IVIG may be useful to dampen hyperacute rejection.
A E Fiane; V Videm; O J Mellbye; A Foerster; O R Geiran; I P Gladhaug; J L Svennevig; T E Mollnes
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Scandinavian journal of immunology     Volume:  47     ISSN:  0300-9475     ISO Abbreviation:  Scand. J. Immunol.     Publication Date:  1998 Jun 
Date Detail:
Created Date:  1998-07-30     Completed Date:  1998-07-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0323767     Medline TA:  Scand J Immunol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  568-74     Citation Subset:  IM    
Department of Surgery A, University of Oslo, The National Hospital, Norway.
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MeSH Terms
Complement Activation
Complement C1r / metabolism
Complement C1s / metabolism
Complement C3 Convertase, Alternative Pathway
Complement C3b / metabolism
Complement C4b / metabolism
Graft Survival*
Immunoglobulins, Intravenous / pharmacology*
Kidney Transplantation / immunology*
Models, Biological
Peptide Fragments / metabolism
Transplantation, Heterologous
Reg. No./Substance:
0/Immunoglobulins, Intravenous; 0/Peptide Fragments; 80295-43-8/Complement C3b; 80295-50-7/Complement C4b; EC C1r; EC C1s; EC C3 Convertase, Alternative Pathway

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