| Immunoglobulin isotypes of anti-myeloperoxidase and anti-lactoferrin antibodies in patients with collagen diseases. | |
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MedLine Citation:
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PMID: 10939715 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To investigate the prevalence and clinical relevance of immunoglobulin (Ig) isotypes of antimyeloperoxidase (MPO) and antilactoferrin (LF) antibodies in collagen diseases, enzyme-linked immunosorbent assay was employed to detect the Ig isotypes of both antibodies. The purified proteins of MPO and LF were used as two major representative antigens for anti-neutrophil cytoplasmic antibodies (ANCA) with a perinuclear staining pattern by an indirect immunofluorescent technique. We examined 131 serum samples from 79 patients with rheumatoid arthritis (RA), 32 with systemic lupus erythematosus (SLE), 14 with progressive systemic sclerosis (PSS), 6 with polymyositis/dermatomyositis (PM/DM), and 5 with idiopathic crescentic glomerulonephritis who served as positive controls and 36 healthy subjects who served as controls. A limited number of patients with RA (4-10%), SLE (6-9%), and PSS (7-14%) but not PM/DM showed positive IgG or IgA anti-MPO antibody (MPO-ANCA) but not IgM MPO-ANCA. However, 10-20% of RA, 40-60% of SLE, 20-36% of PSS but none of the PM/DM patients showed positive IgG, IgA, or IgM anti-LF antibody (LF-ANCA). MPO- and LF-ANCA positivity in RA patients was correlated with markers of disease activity such as the erythrocyte sedimentation rate, C-reactive protein, and serum Ig levels. IgG LF-ANCA but not MPO-ANCA positivity in SLE patients also was correlated with the disease activity index but not with clinical features. Neither MPO- nor LF-ANCA positivity in PSS patients was correlated with any clinical features. Overall, both MPO- and LF-ANCA were found mainly in RA, SLE, and PSS patients but not in PM/DM patients. The Ig isotypes of MPO- and LF-ANCA frequently belonged to both IgG and IgA and rarely to the IgM class. Both MPO- and LF-ANCA positivity reflected disease activity in RA and SLE rather than specific organ involvement. |
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Authors:
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H Chikazawa; K Nishiya; A Matsumori; K Hashimoto |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of clinical immunology Volume: 20 ISSN: 0271-9142 ISO Abbreviation: J. Clin. Immunol. Publication Date: 2000 Jul |
Date Detail:
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Created Date: 2000-11-21 Completed Date: 2001-02-01 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 8102137 Medline TA: J Clin Immunol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 279-86 Citation Subset: IM |
Affiliation:
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Second Department of Internal Medicine, Kochi Medical School, Nankoku City, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aged Aged, 80 and over Antibodies, Antineutrophil Cytoplasmic / blood, immunology* Antibody Specificity Arthritis, Rheumatoid / immunology Autoantigens / immunology* Autoimmune Diseases / immunology* Collagen Diseases / immunology* Dermatomyositis / immunology Enzyme-Linked Immunosorbent Assay Female Glomerulonephritis / immunology Humans Immunoglobulin A / blood, immunology Immunoglobulin G / blood, immunology Immunoglobulin Isotypes / blood* Immunoglobulin M / blood, immunology Lactoferrin / immunology* Lupus Erythematosus, Systemic / immunology Male Middle Aged Organ Specificity Peroxidase / immunology* Polymyositis / immunology Scleroderma, Systemic / immunology |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Antineutrophil Cytoplasmic; 0/Autoantigens; 0/Immunoglobulin A; 0/Immunoglobulin G; 0/Immunoglobulin Isotypes; 0/Immunoglobulin M; 0/Lactoferrin; EC 1.11.1.7/Peroxidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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