Document Detail

Immunoglobulin gene rearrangement analysis in composite hodgkin disease and large B-cell lymphoma: evidence for receptor revision of immunoglobulin heavy chain variable region genes in Hodgkin-Reed-Sternberg cells?
MedLine Citation:
PMID:  11854595     Owner:  NLM     Status:  MEDLINE    
Immunoglobulin heavy chain gene (IgH) rearrangement was studied in a patient showing the occurrence of classical Hodgkin disease and large B-cell lymphoma (LBCL) in the same lymph node. The VHDHJH region was amplified by polymerase chain reaction, the template being the DNA extracted from single Hodgkin and Reed-Sternberg and LBCL cells, microdissected on hematoxylin-eosin-stained sections by laser capture. A repeated VH4DH3JH4 segment was found in Reed-Sternberg cells, whereas a repeated VH3DH3JH4 segment was observed in LBCL cells. Rearranged VH genes carried somatic mutations in both populations, indicating a common germinal center cell origin. The IgH rearrangement found in clonally related Reed-Sternberg cells differed from the one of LBCL cells in the VH region but showed the same JH and DH segments with no variation from the respective germline sequence. The DH-JH junction is the first immunoglobulin gene segment rearranged in precursor B cells. Because the possibility of secondary Ig gene rearrangement in peripheral lymphoid organs has recently been reported, in the patient described here Reed-Sternberg and LBCL cells might originate from a common precursor in which secondary VH replacement took place during the germinal center reaction, giving rise to two different clonally related lymphomas.
Cristiana Bellan; Stefano Lazzi; Maurizio Zazzi; Anna Vittoria Lalinga; Nazareno Palummo; Piero Galieni; Teresa Marafioti; Tiziana Tonini; Caterina Cinti; Lorenzo Leoncini; Stefano A Pileri; Piero Tosi
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Publication Detail:
Type:  Case Reports; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Diagnostic molecular pathology : the American journal of surgical pathology, part B     Volume:  11     ISSN:  1052-9551     ISO Abbreviation:  Diagn. Mol. Pathol.     Publication Date:  2002 Mar 
Date Detail:
Created Date:  2002-02-20     Completed Date:  2002-04-25     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9204924     Medline TA:  Diagn Mol Pathol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2-8     Citation Subset:  IM    
Institute of Pathologic Anatomy and Histopathology, University of Siena, Italy.
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MeSH Terms
DNA, Neoplasm / analysis
Gene Rearrangement, B-Lymphocyte, Heavy Chain / genetics*
Hodgkin Disease / genetics*,  metabolism,  pathology
Immunoglobulin Variable Region / genetics*,  metabolism
Lymph Nodes / metabolism,  pathology
Lymphoma, B-Cell / genetics,  metabolism,  pathology
Lymphoma, Large B-Cell, Diffuse / genetics,  metabolism,  pathology
Lymphoma, Non-Hodgkin / genetics*,  metabolism,  pathology
Polymerase Chain Reaction
Receptors, Antigen, B-Cell / genetics*,  metabolism
Reed-Sternberg Cells / immunology*,  metabolism,  pathology
Sequence Analysis, DNA
Tumor Markers, Biological / metabolism
Reg. No./Substance:
0/DNA, Neoplasm; 0/Immunoglobulin Variable Region; 0/Receptors, Antigen, B-Cell; 0/Tumor Markers, Biological

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