Document Detail

Immunoglobulin coating of faecal bacteria in inflammatory bowel disease.
MedLine Citation:
PMID:  15201580     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: An inappropriate mucosal immune response to the commensal bacterial flora may play a role in the pathogenesis of inflammatory bowel disease (IBD). In this study we determined the percentage of immunoglobulin-coated bacteria in the stools of patients and controls. METHODS: Faecal samples were obtained from 18 patients with IBD (one sample during exacerbation and one shortly after remission was achieved), 15 healthy volunteers, eight infectious colitis patients, and 13 IBD patients in long-term remission. Bacterial immunoglobulin coating was determined by flow-cytometry analysis. Faecal alpha-1-antitrypsin concentrations were determined by radial immune diffusion. RESULTS: IBD patients had 69 +/- 19% immunoglobulin A (IgA)-, 56 +/- 32% immunoglobulin G (IgG)- and 56 +/- 29% immunoglobulin M (IgM)-coated bacteria in their faeces. Healthy controls had less immunoglobulin coating, respectively 36 +/- 12%, 11 +/- 4% and 11 +/- 7%. Infectious colitis patients had 57 +/- 14% IgA, 31 +/- 13% IgG, and 42 +/-16% IgM; however, they had higher faecal alpha-1-antitrypsin concentrations than IBD patients. Shortly after remission, IBD patients had 65 +/- 20% IgA, 32 +/- 18% IgG and 40 +/- 21% IgM. Long-term-remission IBD patients had normal IgG and IgM but increased IgA (50 +/- 16%) coating. CONCLUSIONS: Compared with healthy controls, patients with IBD had an increased percentage of immunoglobulin-coated faecal anaerobic bacteria, both in active disease and shortly after remission. These results support the concept that there may be a breakdown of mucosal tolerance to the commensal gut flora in IBD.
Laurens A van der Waaij; Frans G M Kroese; Annie Visser; Gerardus F Nelis; Bram D Westerveld; Peter L M Jansen; John O Hunter
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of gastroenterology & hepatology     Volume:  16     ISSN:  0954-691X     ISO Abbreviation:  Eur J Gastroenterol Hepatol     Publication Date:  2004 Jul 
Date Detail:
Created Date:  2004-06-17     Completed Date:  2005-03-17     Revised Date:  2009-10-16    
Medline Journal Info:
Nlm Unique ID:  9000874     Medline TA:  Eur J Gastroenterol Hepatol     Country:  England    
Other Details:
Languages:  eng     Pagination:  669-74     Citation Subset:  IM    
Department of Internal Medicine--Gastroenterology Section, University of Groningen, Groningen, The Netherlands.
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MeSH Terms
Antibodies, Bacterial / analysis*
Bacteria / immunology*
Colitis / immunology,  microbiology
Feces / enzymology,  microbiology*
Immune Tolerance
Immunity, Mucosal
Immunoglobulin A / analysis
Immunoglobulin G / analysis
Immunoglobulin M / analysis
Immunoglobulins / analysis*
Inflammatory Bowel Diseases / immunology*,  microbiology
Middle Aged
Remission Induction
Symbiosis / immunology
alpha 1-Antitrypsin / analysis
Reg. No./Substance:
0/Antibodies, Bacterial; 0/Immunoglobulin A; 0/Immunoglobulin G; 0/Immunoglobulin M; 0/Immunoglobulins; 0/SERPINA1 protein, human; 0/alpha 1-Antitrypsin

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