Document Detail

Immunoglobulin G-dependent changes in basophil allergen threshold sensitivity during birch pollen immunotherapy.
MedLine Citation:
PMID:  20528883     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: The effects of pollen immunotherapy on effector cells of allergic inflammation, such as mast cells and basophils, are poorly understood. OBJECTIVE: For this reason, we conducted an open study on basophil allergen threshold sensitivity during birch pollen immunotherapy. METHODS: Basophil sensitivity was measured by CD63 flow cytometry in 14 patients with moderate-severe intermittent birch pollen rhinitis using four log allergen concentrations. In nine patients, we analysed the basophil sensitivity before and during treatment with subcutaneous birch immunotherapy (perennial scheme with allergoid). We also included eight birch-allergic donor subjects for IgG inhibition experiments and eight control subjects. RESULTS: There was a decrease in basophil allergen threshold sensitivity after 2, 3, and 5 months of immunotherapy. This decrease was correlated with an improvement in patients' symptoms measured on a visual analogue scale. The serum obtained after immunotherapy induced a significant decrease in allergen threshold sensitivity in donor birch-allergic basophils. This inhibition was not observed after IgG depletion from the serum. CONCLUSIONS: In this study, we showed that birch immunotherapy-induced IgG antibodies are associated with a reduction in basophil allergen threshold sensitivity. Further studies are needed to show whether the changes in basophil sensitivity are of clinical relevance in pollen immunotherapy.
N Lalek; M Kosnik; M Silar; P Korosec
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Publication Detail:
Type:  Journal Article     Date:  2010-05-31
Journal Detail:
Title:  Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology     Volume:  40     ISSN:  1365-2222     ISO Abbreviation:  Clin. Exp. Allergy     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-07-23     Completed Date:  2010-11-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8906443     Medline TA:  Clin Exp Allergy     Country:  England    
Other Details:
Languages:  eng     Pagination:  1186-93     Citation Subset:  IM    
University Clinic of Respiratory and Allergic Diseases, Golnik, Slovenia.
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MeSH Terms
Allergens / immunology
Basophils / immunology*
Betula / immunology
Cell Separation
Desensitization, Immunologic*
Flow Cytometry
Immunoglobulin G / immunology*
Middle Aged
Rhinitis, Allergic, Seasonal / blood,  immunology*
Young Adult
Reg. No./Substance:
0/Allergens; 0/Immunoglobulin G

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