| Immunogenicity of rat renal allograft nephron components in vivo. Lack of correlation between MHC expression and immunogenic potential. | |
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MedLine Citation:
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PMID: 2114679 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cultured rat nephron components--i.e., tubular cells, glomerular mesangial cells, and glomerular epithelial cells--were compared with cultured rat heart endothelial cells for their in vivo immunogenicity using the primed rejection assay (PRA). Gamma-interferon (gIFN) was used to regulate the class I and class II MHC antigen expression of the cells tested. The heart endothelial cells proved to be the only cell type capable of inducing a maximal rejection response in the native state. This was achieved with a cell number of 10(6) when the survival of a subsequent heart allograft from a relevant donor was reduced from 5 to 3 days. All kidney nephron components proved to be immunogenic in PRA but to a far lesser extent than the endothelial cells. A reduction of graft survival from 5 to 4 days was achieved with a cell number of 10(6) cells, and no immunogenic effect (with the exception of mesangial cells contaminated by a small number of macrophages) was observed in smaller cell numbers. The gIFN-treated endothelial cells were more potent than untreated endothelial cells. They reduced the graft survival to 4 days with a cell number of 10(3) and caused a maximal reduction of the survival to 3 days with a cell number of 10(5). The nephron components failed to increase their immunogenicity after 3-day gIFN treatment, regardless of a high increase in their class I and class II expression rates. The study suggests that, in contrast to the endothelial cells, none of the nephron components are able to act as an antigen-presenting cell on their own. |
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Authors:
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J Halttunen |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Transplantation Volume: 50 ISSN: 0041-1337 ISO Abbreviation: Transplantation Publication Date: 1990 Jul |
Date Detail:
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Created Date: 1990-08-13 Completed Date: 1990-08-13 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0132144 Medline TA: Transplantation Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 150-5 Citation Subset: IM |
Affiliation:
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Transplantation Laboratory, University of Helsinki, Finland. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antigen-Presenting Cells / physiology Cells, Cultured Graft Rejection Histocompatibility Antigens Class I / immunology* Histocompatibility Antigens Class II / immunology* Interferon-gamma / pharmacology Kidney Transplantation* Nephrons / immunology* Rats Rats, Inbred Strains Transplantation, Homologous |
| Chemical | |
Reg. No./Substance:
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0/Histocompatibility Antigens Class I; 0/Histocompatibility Antigens Class II; 82115-62-6/Interferon-gamma |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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