| Immunogenicity and protective efficacy of two recombinant pseudorabies viruses expressing Toxoplasma gondii SAG1 and MIC3 proteins. | |
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MedLine Citation:
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PMID: 21632181 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Toxoplasma gondii is one of the most common parasitic pathogens in humans and warm-blooded animals, causing toxoplasmosis. One of the efficient ways to control this disease is immunization. In this study, two recombinant pseudorabies virus (PRV) expressing TgSAG1 (rPRV-SAG1) and TgMIC3 (rPRV-MIC3) based on the PRV vaccine strain were developed by homologous recombination and used for immunizing BALB/c mice. Ninety BALB/c mice were randomly divided into five groups including four experimental groups (inoculated twice in 4 weeks interval with PRV TK-/gG-/EGFP+, rPRV-SAG1, rPRV-MIC3, rPRV-SAG1+rPRV-MIC3, respectively) and one control group (inoculated with medium). All mice vaccinated with rPRV developed a high level of specific antibody responses against T. gondii lysate antigen (TLA), a strong increase of the splenocyte proliferative response, and significant levels of IFN-γ and IL-2 production. These results demonstrated that rPRV could induce significant humoral and cellular Th1 immune responses. Moreover, rPRV immunization induced partial protection against a lethal challenge with T. gondii RH strain, and neutralizing antibodies against PRV in a BALB/c mouse model. The mice immunized with the rPRV-SAG1 and rPRV-MIC3 cocktail could develop higher T. gondii-specific IgG antibodies and lymphocyte proliferative responses and conferred more efficient protection against T. gondii challenge. These results suggested that expression of protective antigens of T. gondii in PRV is a novel approach towards the development of a vaccine against both animal pseudorabies and toxoplasmosis. |
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Authors:
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Hao Nie; Rui Fang; Bing-Qing Xiong; Li-Xia Wang; Min Hu; Yan-Qin Zhou; Jun-Long Zhao |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-5-5 |
Journal Detail:
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Title: Veterinary parasitology Volume: - ISSN: 1873-2550 ISO Abbreviation: - Publication Date: 2011 May |
Date Detail:
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Created Date: 2011-6-2 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7602745 Medline TA: Vet Parasitol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011 Elsevier B.V. All rights reserved. |
Affiliation:
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State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Sizhishan Street, Hubei, Wuhan 430070, PR China; Pathogenic Biology Department of Medical School, Yangtze University, Hubei, Jingzhou 434023, PR China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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