| Immunogenicity and efficacy of immunodeficiency virus-like particles pseudotyped with the G protein of vesicular stomatitis virus. | |
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MedLine Citation:
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PMID: 16616946 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Vaccination with exogenous antigens such as recombinant viral proteins, immunodeficiency virus-derived whole inactivated virus particles, or virus-like particles (VLP) has generally failed to provide sufficient protection in animal models for AIDS. Pseudotyping VLPs with the vesicular stomatitis virus G protein (VSV-G), which is known to mediate entry into dendritic cells, might allow more efficient stimulation of immune responses. Therefore, we pseudotyped noninfectious immunodeficiency virus-like particles with VSV-G and carried out a preliminary screen of their immunogenicity and vaccination efficacy. Incorporation of VSV-G into HIV-1 VLPs led to hundred-fold higher antibody titers to HIV-1 Gag and enhancement of T cell responses in mice. Repeated vaccination of rhesus monkeys for 65 weeks with VSV-G pseudotyped simian immunodeficiency virus (SIV)-like particles (VLP[G]) provided initial evidence for efficient suppression of viral load after mucosal challenge with the SIVmac239 virus. Challenge of monkeys after a 28 week vaccination regimen with VLP[G] led to a reduction in peak viremia, but persistent suppression of viral load was not achieved. Due to limitations in the number of animals available for this study, improved efficacy of VSV-G pseudotyped VLPs in nonhuman primates could not be demonstrated. However, mouse experiments revealed that pseudotyping of VLPs with fusion-competent VSV-G clearly improves their immunogenicity. Additional strategies, particularly adjuvants, should be considered to provide greater protection against a challenge with pathogenic immunodeficiency virus. |
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Authors:
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Seraphin Kuate; Christiane Stahl-Hennig; Heribert Stoiber; Godwin Nchinda; Anja Floto; Monika Franz; Ulrike Sauermann; Simon Bredl; Ludwig Deml; Ralf Ignatius; Steve Norley; Paul Racz; Klara Tenner-Racz; Ralph M Steinman; Ralf Wagner; Klaus Uberla |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2006-04-17 |
Journal Detail:
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Title: Virology Volume: 351 ISSN: 0042-6822 ISO Abbreviation: Virology Publication Date: 2006 Jul |
Date Detail:
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Created Date: 2006-07-17 Completed Date: 2006-09-06 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0110674 Medline TA: Virology Country: United States |
Other Details:
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Languages: eng Pagination: 133-44 Citation Subset: IM |
Affiliation:
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Department of Molecular and Medical Virology, Ruhr-University Bochum, D-44780 Bochum, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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AIDS Vaccines
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administration & dosage,
immunology* Animals Cell Line Drug Administration Schedule HIV Antibodies / blood Humans Macaca mulatta / immunology Membrane Glycoproteins / genetics, immunology* Mice RNA, Viral / blood Time Factors Vesicular stomatitis Indiana virus* Viral Envelope Proteins / genetics, immunology* Viral Load Virion / genetics, immunology |
| Grant Support | |
ID/Acronym/Agency:
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AI40874/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/AIDS Vaccines; 0/G protein, vesicular stomatitis virus; 0/HIV Antibodies; 0/Membrane Glycoproteins; 0/RNA, Viral; 0/Viral Envelope Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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