Document Detail


Immunofluorescent study after transient transfection of myoblast cells with the plasmid PSV40-beta-globin.
MedLine Citation:
PMID:  9080679     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The level of transfection of myoblast C-2/C-12 cells with the plasmid PSV40-beta-globin, using the immunofluorescence assay, was investigated. Myoblasts, which are non-terminally differentiated cells, were chosen since it is known that terminally differentiated myotubes are very poorly transfected. The results showed that myoblast cells of the C-2/C-12 cell line were readily transfected within 24 h with the PSV40-beta-globin gene. The immunofluorescence assay showed the nucleoplasmic localization of the SV40 large T antigen, and indicated the possibility that some fraction of it might be localized in the cytoplasm.
Authors:
G L Pironcheva; G Russev
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cytobios     Volume:  86     ISSN:  0011-4529     ISO Abbreviation:  Cytobios     Publication Date:  1996  
Date Detail:
Created Date:  1997-05-19     Completed Date:  1997-05-19     Revised Date:  2013-04-05    
Medline Journal Info:
Nlm Unique ID:  0207227     Medline TA:  Cytobios     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  243-6     Citation Subset:  IM    
Affiliation:
Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia, Bulgaria.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, Polyomavirus Transforming / biosynthesis*,  genetics
Calcium Phosphates
Cell Differentiation
Cell Nucleus / metabolism
Cytoplasm / metabolism
Fluorescent Antibody Technique, Indirect*
Genetic Vectors*
Globins / biosynthesis*,  genetics
Mice
Microscopy, Fluorescence
Muscles / cytology*,  metabolism
Rabbits
Rats
Recombinant Fusion Proteins / biosynthesis*,  genetics
Simian virus 40 / genetics*
Transfection*
Chemical
Reg. No./Substance:
0/Antigens, Polyomavirus Transforming; 0/Calcium Phosphates; 0/Recombinant Fusion Proteins; 0/alpha-tricalcium phosphate; 0/monocalcium phosphate; 0/tetracalcium phosphate; 9004-22-2/Globins; 97Z1WI3NDX/calcium phosphate; L11K75P92J/dicalcium phosphate anhydrous

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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