| Immunofluorescence of phenobarbital inducible cytochrome P-450 in the hepatic lobule of normal and phenobarbital-treated rats. | |
| | |
MedLine Citation:
|
PMID: 6815045 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The localization of the form of cytochrome P-450 that is induced by phenobarbital was studied by direct immunofluorescence in the hepatocytes of rats pretreated with phenobarbital in comparison with saline-treated rats. Specific fluorescence was seen in the hepatocyte cytoplasm in saline- and phenobarbital-treated rats; a more concentrated halo of fluorescence was detected surrounding the nuclei in the centrilobular zones after phenobarbital treatment. In the saline-treated rats, fluorescence was barely discernible but slightly more intense in the centrilobular than perilobular zones. In phenobarbital-treated rats, fluorescence was much more intense, with a similar but much greater difference between the centrilobular and perilobular zones. The tissue distribution and induction site of this component of the cytochrome P-450-dependent microsomal system may be relevant to the site of drug toxicity and the development of chemical carcinogenesis. |
| | |
Authors:
|
K Ohnishi; A Mishima; K Okuda |
Related Documents
:
|
7681935 - Effects of glucosinolate breakdown products on the hepatic biotransformation system in ... 2918465 - Stereoselectivity of styrene oxidation in microsomes and in purified cytochrome p-450 e... 1472535 - Alteration of cytochrome p-450 2c11-dependent testosterone metabolism in gunn rat liver. 3705085 - Rat lung and liver cytochrome p-450 isozymes involved in the hydroxylation of m-xylene. 2947745 - Atrial natriuretic factor. 1921635 - Response of the stria vascularis to corticosteroids. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Hepatology (Baltimore, Md.) Volume: 2 ISSN: 0270-9139 ISO Abbreviation: Hepatology Publication Date: 1982 Nov-Dec |
Date Detail:
|
Created Date: 1983-01-07 Completed Date: 1983-01-07 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 8302946 Medline TA: Hepatology Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 849-55 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cytochrome P-450 Enzyme System / analysis*, biosynthesis Enzyme Induction Fluorescent Antibody Technique Immunodiffusion Liver / drug effects, enzymology* Male Phenobarbital / pharmacology* Rats Rats, Inbred Strains |
| Chemical | |
Reg. No./Substance:
|
50-06-6/Phenobarbital; 9035-51-2/Cytochrome P-450 Enzyme System |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Nitroglycerin improves the hemodynamic response to vasopressin in portal hypertension.
Next Document: Valproate-induced hepatic steatogenesis in rats.