Document Detail


Immunoenzymatic detection of staphylococcal enterotoxins: international interlaboratory study.
MedLine Citation:
PMID:  8823918     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An international interlaboratory study was performed by 13 laboratories to validate a commercially available, rapid enzyme immunoassay for detection of staphylococcal enterotoxins A, B, C, D, and E in foods. The 5 enterotoxin serotypes were detected at a level of 0.5 ng/g in mushrooms and ravioli, 0.8 ng/g in meat, 1 ng/mL in milk, and 1.5 ng/g in raw milk cheese when these foods were artificially contaminated with enterotoxin A. Enterotoxins A, B, C, D, or E were also detected in culture supernatants with no protein A interference when normal rabbit serum was used. This method was validated by the French Normalization Agency for the identification of staphylococcal enterotoxins in foods and culture fluids.
Authors:
C Lapeyre; M N de Solan; X Drouet
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Publication Detail:
Type:  Comparative Study; Journal Article; Multicenter Study    
Journal Detail:
Title:  Journal of AOAC International     Volume:  79     ISSN:  1060-3271     ISO Abbreviation:  J AOAC Int     Publication Date:    1996 Sep-Oct
Date Detail:
Created Date:  1996-12-02     Completed Date:  1996-12-02     Revised Date:  2008-03-17    
Medline Journal Info:
Nlm Unique ID:  9215446     Medline TA:  J AOAC Int     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1095-101     Citation Subset:  IM    
Affiliation:
Centre National D'Etudes Vétérinaires et Alimentaires, CNEVA Paris-LCHA, France.
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MeSH Terms
Descriptor/Qualifier:
Antibody Specificity
Culture Media
Enterotoxins / analysis*,  biosynthesis
Food Analysis / standards
Food Contamination
Immunoenzyme Techniques
International Cooperation
Reagent Kits, Diagnostic
Reference Standards
Reproducibility of Results
Staphylococcal Food Poisoning / diagnosis*,  prevention & control
Staphylococcus aureus / metabolism*
Chemical
Reg. No./Substance:
0/Culture Media; 0/Enterotoxins; 0/Reagent Kits, Diagnostic

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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