Document Detail


Immunoenhancing effects of bovine glycomacropeptide and its derivatives on the proliferative response and phagocytic activities of human macrophagelike cells, U937.
MedLine Citation:
PMID:  15113179     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The immunomodulatory effect of GMP and its derivates on the cell proliferative response of human macrophagelike cell, U937, and its effect on phagocytic activities via incorporation of fluorescence beads were studied. GMP was found to be a potent immunoenhancer at low concentrations, significantly enhancing the proliferation and phagocytic activities of U937. The modulatory function could be radically altered by enzymatic treatments. Pepsin digestion significantly enhanced the degree of cell proliferation and phagocytic activities, whereas trypsin had no significant effect. The immunoenhancing effects decreased significantly after sialidase treatment; however, more than 70% of activity was retained after treatment. GMP with different carbohydrate chains was shown to possess different modulatory capabilities. Sialic acid-rich GMP fractions showed an enhanced response. These findings indicate that both the carbohydrate chains compositions, including the terminal sialic acids and the polypeptide portions of GMP, are essential for the stimulatory effects of GMP on cell proliferation and phagocytic activities of U937.
Authors:
Esther W Y Li; Yoshinori Mine
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  52     ISSN:  0021-8561     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2004 May 
Date Detail:
Created Date:  2004-04-28     Completed Date:  2004-06-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2704-8     Citation Subset:  IM    
Affiliation:
Department of Food Science, University of Guelph, Guelph, Ontario N1G 2W1, Canada.
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MeSH Terms
Descriptor/Qualifier:
Animals
Carbohydrates / analysis
Caseins / chemistry*
Cattle
Cell Division / drug effects*
Cell Line
Dose-Response Relationship, Drug
Humans
Macrophages / drug effects*,  immunology
Milk / chemistry
Neuraminidase / metabolism
Pepsin A / metabolism
Peptide Fragments / chemistry,  metabolism,  pharmacology*
Phagocytosis / drug effects*
Sialic Acids / analysis,  metabolism,  pharmacology
Structure-Activity Relationship
Trypsin / metabolism
Chemical
Reg. No./Substance:
0/Carbohydrates; 0/Caseins; 0/Peptide Fragments; 0/Sialic Acids; EC 3.2.1.18/Neuraminidase; EC 3.4.21.4/Trypsin; EC 3.4.23.1/Pepsin A

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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