Document Detail

Immunoelectronmicroscopy of metaphase chromosomes.
MedLine Citation:
PMID:  6179900     Owner:  NLM     Status:  MEDLINE    
A method for the preparation of ultrathin sections of metaphase chromosomes is described. This method was applied to human metaphase chromosomes, which were immunocytochemically stained with anti-DNA and anti-ribonucleoprotein antibodies, derived from patients with auto-immune disease. Conventionally prepared metaphase spreads as well as cytocentrifuge preparations of chromosome suspensions were studied. The results indicate that the ultrastructure of chromosomes and the immunoreactivity of chromosomal constituents are influenced by the applied preparation methods. In comparison with whole mount preparations, ultrathin sections of immunostained chromosomes allow higher resolution and more precise localization of immunoreactive sites within the chromosomal structure.
F T Bosman; P K Nakane
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Histochemistry     Volume:  74     ISSN:  0301-5564     ISO Abbreviation:  Histochemistry     Publication Date:  1982  
Date Detail:
Created Date:  1982-10-21     Completed Date:  1982-10-21     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0411300     Medline TA:  Histochemistry     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  341-6     Citation Subset:  IM    
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MeSH Terms
Antibodies, Antinuclear*
Antigen-Antibody Reactions
Chromosomes, Human / ultrastructure*
Microscopy, Electron
Staining and Labeling
Reg. No./Substance:
0/Antibodies, Antinuclear

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