| Immunoelectron microscopic visualization of the transcytosis of low density lipoproteins in perfused rat arteries. | |
| | |
MedLine Citation:
|
PMID: 2743992 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
A postembedding labeling technique was employed to visualize human native low density lipoproteins (LDL) during transcytosis in rat arterial endothelium. For this purpose human LDL was perfused through rat vasculature before fixation and processing for immunoelectron microscopy. The LDL particles were located on sections by anti-human apolipoprotein B-100 (LDL) antibodies and secondary antibodies or protein-A conjugated to 10-nm colloidal gold. LDL molecules were seen in plasmalemmal vesicles as well as in the subendothelial space. No colloidal gold was found in the intercellular junctions. Perfusion with reductively methylated LDL, which cannot bind to the LDL receptor, gave a similar labeling pattern, indicating that transcytosis of LDL via plasmalemmal vesicles is most likely receptor independent. Furthermore, the passage of LDL through intact vascular endothelium is a vesicular transport rather than an intercellular diffusion process. |
| | |
Authors:
|
I Snelting-Havinga; M Mommaas; V W Van Hinsbergh; M R Daha; W T Daems; B J Vermeer |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: European journal of cell biology Volume: 48 ISSN: 0171-9335 ISO Abbreviation: Eur. J. Cell Biol. Publication Date: 1989 Feb |
Date Detail:
|
Created Date: 1989-08-18 Completed Date: 1989-08-18 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 7906240 Medline TA: Eur J Cell Biol Country: GERMANY, WEST |
Other Details:
|
Languages: eng Pagination: 27-36 Citation Subset: IM |
Affiliation:
|
Department of Dermatology, Leiden University, The Netherlands. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Arteries / cytology, metabolism*, ultrastructure Endothelium, Vascular / metabolism, ultrastructure Humans Immunohistochemistry Lipoproteins, LDL / metabolism* Male Microscopy, Electron / methods Perfusion Rats Rats, Inbred Strains Receptors, LH / metabolism, physiology |
| Chemical | |
Reg. No./Substance:
|
0/Lipoproteins, LDL; 0/Receptors, LH |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: A colloidal gold labeling technique for the direct determination of the surface area of eukaryotic c...
Next Document: Biochemical, kinetic and cytochemical approaches resolve Golgi subcompartments of IgM-secreting rat ...