Document Detail


Immunoelectron microscopic localization of D-amino acid oxidase in rat kidney and liver.
MedLine Citation:
PMID:  2885296     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The intracellular localization of D-amino acid oxidase in rat kidney and liver has been investigated using the indirect immunogold postembedding technique. Different fixation and embedding conditions for optimal preservation of antigenicity and fine structure have been tested. Immunolabelling was possible only in tissues embedded in polar resins (glycol methacrylate and Lowicryl K4M). In kidney the enzyme was demonstrable only in the peroxisomes of the proximal tubule, where it was associated with the peroxisome core. The enzyme was present in all the peroxisomes of the proximal tubule and appeared to be codistributed with catalase. Control experiments and quantitative analysis confirmed the specificity of the D-amino acid oxidase immunolocalization. All the other cells in kidney failed to demonstrate any labelling. In liver, the immunolabelling was present in the matrix of the hepatocyte peroxisomes, whereas no traces of the enzyme were found in the nucleoid. The intensity of the immunolabelling in liver peroxisomes was lower than in kidney. No specific labelling was observed in cells other than hepatocytes.
Authors:
M E Perotti; E Gavazzi; L Trussardo; N Malgaretti; B Curti
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Histochemical journal     Volume:  19     ISSN:  0018-2214     ISO Abbreviation:  Histochem. J.     Publication Date:  1987 Mar 
Date Detail:
Created Date:  1987-08-10     Completed Date:  1987-08-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0163161     Medline TA:  Histochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  157-69     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
D-Amino-Acid Oxidase / metabolism*
Histocytochemistry
Immunodiffusion
Kidney / enzymology*,  ultrastructure
Kidney Cortex / enzymology
Kidney Tubules, Proximal / enzymology
Liver / enzymology*,  ultrastructure
Male
Microbodies / enzymology,  ultrastructure
Microscopy, Electron
Rats
Rats, Inbred Strains
Chemical
Reg. No./Substance:
EC 1.4.3.3/D-Amino-Acid Oxidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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