Document Detail

Immunodiagnoses of community-acquired pneumonia in childhood.
MedLine Citation:
PMID:  9283122     Owner:  NLM     Status:  MEDLINE    
A diagnosis of bacterial pneumonia requires isolation of the pathogen from blood, lung or tracheal aspirate; however, cultures of blood and pleural fluid samples are usually insensitive. Thus, in the majority of patients the etiology is rarely determined. A total of 840 pleural fluid effusion samples from children with clinical and laboratory diagnoses of acute bacterial pneumonia were evaluated by Dot-ELISA. This method was standardized in order to detect polysaccharide capsular bacterial antigen in pleural fluid samples previously treated with 0.1 M EDTA and dotted on nitrocellulose membrane strips. Pneumococcal omniserum and H. influenzae type b antiserum diluted 1:200 were employed for detection of S. pneumoniae and H. influenzae type b antigens, respectively. Dot-ELISA showed relative indices of 0.913 for sensitivity and 0.552 for specificity, and a total positivity of 75.6 per cent, being 53.21 per cent for S. pneumoniae and of 22.38 per cent for H. influenzae.
H I Requejo; M L Guerra; M Dos Santos; A M Cocozza
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Journal of tropical pediatrics     Volume:  43     ISSN:  0142-6338     ISO Abbreviation:  J. Trop. Pediatr.     Publication Date:  1997 Aug 
Date Detail:
Created Date:  1997-10-09     Completed Date:  1997-10-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8010948     Medline TA:  J Trop Pediatr     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  208-12     Citation Subset:  IM    
Instituto Adolfo Lutz, Department of Immunology, São Paulo, Brazil.
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MeSH Terms
Antigens, Bacterial / analysis*
Child, Preschool
Community-Acquired Infections
Enzyme-Linked Immunosorbent Assay
False Positive Reactions
Haemophilus Infections / diagnosis
Haemophilus influenzae / isolation & purification
Infant, Newborn
Pleural Effusion / microbiology*
Pneumonia, Pneumococcal / diagnosis*
Polysaccharides, Bacterial / analysis*
Sensitivity and Specificity
Streptococcus pneumoniae / isolation & purification
Reg. No./Substance:
0/Antigens, Bacterial; 0/Polysaccharides, Bacterial

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