Document Detail


Immunodetection and localization of protein(s) related to retinal S-antigen (arrestin) in kidney.
MedLine Citation:
PMID:  1300198     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
S-antigen (arrestin) is a cytosolic protein which regulates phototransduction in retinal rods. A protein immunologically related to S-antigen was identified in fractions from soluble extract of bovine kidney enriched by gel filtration or by immunoaffinity chromatography using a polyclonal antibody to retinal S-antigen. On immunoblots, this protein was recognized by a panel of monoclonal antibodies (mAbs S2D2, S1A3 and S9E2) directed against different S-antigen epitopes and displayed the same apparent molecular mass (48 kDa) as retinal S-antigen. All three mAbs revealed a specific immunoreactivity by indirect immunocytochemical technique on rat kidney sections. The three mAbs recognized some but not all glomerular cells, identified as epithelial cells by immunoelectron microscopy using the mAb S9E2. Both mAbs S2D2 and S1A3 gave a diffuse cytoplasmic staining in all tubule cells. Proximal tubule cells exhibited a weak immunoreactivity, whereas distal and collecting tubule cells were strongly labeled. In contrast, the mAb S9E2 immunoreaction was restricted to a cell subpopulation from distal and collecting tubules corresponding to intercalated cells identified by immunoelectron microscopy. With the mAb S9E2, the labeling of proximal tubule cells was localized in the apical region of the cytoplasm. These results suggest that two or more 48-kDa proteins immunologically cross-reactive with retinal S-antigen are present in kidney. The observed pattern of distribution is in keeping with the hypothesis that such proteins could play a role in the regulation of G-protein-related receptors present in renal glomerulus and tubule epithelial cells.
Authors:
M Mirshahi; A Razaghi; A Vandewalle; F Cluzeaud; M Tarraf; J P Faure
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biology of the cell / under the auspices of the European Cell Biology Organization     Volume:  76     ISSN:  0248-4900     ISO Abbreviation:  Biol. Cell     Publication Date:  1992  
Date Detail:
Created Date:  1993-05-24     Completed Date:  1993-05-24     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  8108529     Medline TA:  Biol Cell     Country:  FRANCE    
Other Details:
Languages:  eng     Pagination:  175-84     Citation Subset:  IM    
Affiliation:
INSERM U86, Centre de Recherches Biomédicales des Cordeliers, Paris, France.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Antibodies, Monoclonal / chemistry
Antigen-Antibody Reactions
Antigens / analysis*,  immunology,  ultrastructure
Arrestin
Cattle
Epithelium / chemistry,  immunology,  ultrastructure
Eye Proteins / analysis*,  immunology,  ultrastructure
Kidney Tubules / chemistry*,  immunology,  ultrastructure
Molecular Sequence Data
Rats
Rats, Inbred Lew
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens; 0/Arrestin; 0/Eye Proteins

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