Document Detail

Immunodetection of acrosin during the acrosome reaction of hamster, guinea-pig and human spermatozoa.
MedLine Citation:
PMID:  1341578     Owner:  NLM     Status:  MEDLINE    
Mammalian sperm acrosomes contain a trypsin-like protease called acrosin which causes limited and specific hydrolysis of the extracellular matrix of the mammalian egg, the zona pellucida. Acrosin was localized on hamster, guinea-pig and human sperm using monoclonal and polyclonal antibodies to human acrosin labelled with colloidal gold. This was visualized directly with transmission electron microscopy, and with light and scanning microscopy after silver enhancement of the colloidal gold probe. Four distinct labelling patterns were found during capacitation and the acrosome reaction in hamster and guinea-pig spermatozoa, and three patterns were found in human spermatozoa. In the hamster, acrosin was not detected on the inner acrosomal surface after the completion of the acrosome reaction, thus correlating with the observation that hamster spermatozoa lose the ability to penetrate the zona after the acrosome reaction. With guinea-pig and human spermatozoa, acrosin was still detected after the completion of the acrosome reaction, thus correlating with the observation that acrosome reacted guinea-pig spermatozoa bind to and penetrate the zona pellucida.
C Barros; C Capote; C Perez; J A Crosby; M I Becker; A De Ioannes
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biological research     Volume:  25     ISSN:  0716-9760     ISO Abbreviation:  Biol. Res.     Publication Date:  1992  
Date Detail:
Created Date:  1994-01-27     Completed Date:  1994-01-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9308271     Medline TA:  Biol Res     Country:  CHILE    
Other Details:
Languages:  eng     Pagination:  31-40     Citation Subset:  IM    
Embryology Laboratory, Faculty of Biological Sciences, P. Catholic University of Chile, Santiago.
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MeSH Terms
Acrosin / analysis*
Acrosome / enzymology,  physiology*,  ultrastructure
Guinea Pigs
Microscopy, Immunoelectron
Ovum / physiology*
Sperm-Ovum Interactions*
Zona Pellucida / physiology
Reg. No./Substance:

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