| Immunodetection of acrosin during the acrosome reaction of hamster, guinea-pig and human spermatozoa. | |
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MedLine Citation:
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PMID: 1341578 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Mammalian sperm acrosomes contain a trypsin-like protease called acrosin which causes limited and specific hydrolysis of the extracellular matrix of the mammalian egg, the zona pellucida. Acrosin was localized on hamster, guinea-pig and human sperm using monoclonal and polyclonal antibodies to human acrosin labelled with colloidal gold. This was visualized directly with transmission electron microscopy, and with light and scanning microscopy after silver enhancement of the colloidal gold probe. Four distinct labelling patterns were found during capacitation and the acrosome reaction in hamster and guinea-pig spermatozoa, and three patterns were found in human spermatozoa. In the hamster, acrosin was not detected on the inner acrosomal surface after the completion of the acrosome reaction, thus correlating with the observation that hamster spermatozoa lose the ability to penetrate the zona after the acrosome reaction. With guinea-pig and human spermatozoa, acrosin was still detected after the completion of the acrosome reaction, thus correlating with the observation that acrosome reacted guinea-pig spermatozoa bind to and penetrate the zona pellucida. |
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Authors:
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C Barros; C Capote; C Perez; J A Crosby; M I Becker; A De Ioannes |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biological research Volume: 25 ISSN: 0716-9760 ISO Abbreviation: Biol. Res. Publication Date: 1992 |
Date Detail:
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Created Date: 1994-01-27 Completed Date: 1994-01-27 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9308271 Medline TA: Biol Res Country: CHILE |
Other Details:
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Languages: eng Pagination: 31-40 Citation Subset: IM |
Affiliation:
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Embryology Laboratory, Faculty of Biological Sciences, P. Catholic University of Chile, Santiago. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acrosin
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analysis* Acrosome / enzymology, physiology*, ultrastructure Animals Cricetinae Female Guinea Pigs Male Microscopy, Immunoelectron Ovum / physiology* Sperm-Ovum Interactions* Zona Pellucida / physiology |
| Chemical | |
Reg. No./Substance:
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EC 3.4.21.10/Acrosin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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