| Immunocytochemistry in Europe: results of the European Federation of Cytology Societies (EFCS) inquiry. | |
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MedLine Citation:
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PMID: 21771093 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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F. Schmitt, B. Cochand-Priollet, M. Toetsch, B. Davidson, A. Bondi and P. Vielh Immunocytochemistry in Europe: results of the European Federation of Cytology Societies (EFCS) inquiry Objective: This report describes the results of the first chosen topic of the European Federation of Cytology Societies (EFCS) scientific committee, which concerns the application of immunocytochemistry (ICC) to cytological material. While ICC has become an important ancillary method, not only for diagnosis but also to assess prognostic and predictive factors on cytological material, there are many different methodologies used and the lack of standardization has been criticized. This inquiry aimed first to obtain an overview of the techniques used and second to suggest mechanisms for standardization and quality control. Methods: We report the results of 28 replies from 13 countries to a web-based inquiry into types of specimens, preparations and technical methods. Results: Conventional smears were the preparations most commonly used, followed by cytospins, cell blocks and liquid-based preparations, in that order. Avidin-biotin complex, labelled streptavidin-biotin or peroxidase anti-peroxidase were used in 61% and enhanced-polymer technology in 39%. Automated staining techniques were used by 64%. More than half used the same antibody dilutions as histopathology although only 20% used cell blocks. Conclusion: Reduction of variability by using automation, appropriate controls and customized dilution of antibodies according to different samples could improve the quality of ICC and standardize the techniques, along with quality control and quality assurance measures. |
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Authors:
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F Schmitt; B Cochand-Priollet; M Toetsch; B Davidson; A Bondi; P Vielh |
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Publication Detail:
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Type: JOURNAL ARTICLE |
Journal Detail:
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Title: Cytopathology : official journal of the British Society for Clinical Cytology Volume: 22 ISSN: 1365-2303 ISO Abbreviation: - Publication Date: 2011 Aug |
Date Detail:
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Created Date: 2011-7-20 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9010345 Medline TA: Cytopathology Country: - |
Other Details:
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Languages: ENG Pagination: 238-242 Citation Subset: - |
Copyright Information:
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© 2011 Blackwell Publishing Ltd. |
Affiliation:
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Medical Faculty of Porto, University and Institute of Molecular Pathology and Immunology, University of Porto, Porto, Portugal Department of Pathology and Cytopathology, Lariboisiere Hospital, University Paris 7, Paris, France Institut für Pathologie und Neuropathologie, University of Essen, Essen, Germany Department of Pathology, Norwegian Radium Hospital and Faculty of Medicine, University of Oslo, Oslo, Norway Department of Pathology, Ospedale Maggiore, Bologna, Italy Department of Pathology, Institut de Cancerologie Gustave Roussy, Villejuif, France. |
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