| Immunocytochemical study of retinal diode laser photocoagulation in the rat. | |
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MedLine Citation:
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PMID: 9059277 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIM: To determine the nature of the cellular infiltrate, alterations in cell adhesion molecules, and MHC II antigen expression in the rat retina following diode laser retinal photocoagulation. METHOD: 20 normal Lister rats underwent diode laser photocoagulation of the retina. Frozen sections from eyes enucleated at 0, 1, 5, 13, and 33 days post laser were examined for T cells (R7.3), CD4 T cells (W3/25), activated CD4 T cells (OX-40), CD8 T cells (OX-8), B cells (OX-33), and macrophages (OX-42), MHC II antigen (OX-6), and E-Selectin-1, VCAM-1, and ICAM-1. RESULTS: Retinal diode laser photocoagulation stimulated a wound healing response in the outer retina and choroid. The cellular infiltrate included macrophages and activated CD4 T cells at 13 and 33 days post laser. Glial cells in the inner plexiform and inner nuclear layers expressed MHC II antigen at 24 hours only. ICAM-1 antigen was induced in RPE cells and in Muller cells in the inner retina at all time intervals post laser and intense staining for ICAM-1 was present around intraretinal migrated cells at 13 and 33 days post laser. VCAM-1 antigen expression was induced in the choroidal vascular endothelium and RPE at 13 and 33 days after laser as was E-Selectin-1 antigen expression which was also evident focally at the external limiting membrane in association with migrated cells adjacent to the burn. CONCLUSIONS: These results suggest that alterations in cell adhesion molecules may regulate the migration and activation of retinal pigment epithelium, macrophages and CD4 T cells at the outer blood-retinal barrier and choroid following diode laser photocoagulation of the normal Lister rat retina. |
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Authors:
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P R Richardson; M E Boulton; J Duvall-Young; D McLeod |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The British journal of ophthalmology Volume: 80 ISSN: 0007-1161 ISO Abbreviation: Br J Ophthalmol Publication Date: 1996 Dec |
Date Detail:
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Created Date: 1997-03-20 Completed Date: 1997-03-20 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0421041 Medline TA: Br J Ophthalmol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 1092-8 Citation Subset: IM |
Affiliation:
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University Department of Ophthalmology, Manchester University. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Histocompatibility Antigens Class II / analysis* Immunohistochemistry Laser Coagulation Lymphocytes Postoperative Period Rats Rats, Inbred Strains Retina / pathology, surgery* Wound Healing* |
| Chemical | |
Reg. No./Substance:
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0/Histocompatibility Antigens Class II |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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