Document Detail


Immunocytochemical study of cells in the vitreous of proliferative vitreoretretinopathy.
MedLine Citation:
PMID:  12579654     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: To identify the cellular components of vitreous samples obtained during vitrectomy for proliferative vitreoretinopathy (PVR). METHODS: With the use of three intermediate filament (IF) proteins, vimentin, glial fibrillary acidic protein (GFAP), and cytokeratin (CK), cytocentrifuge slides of 14 fresh vitreous aspirates were detected with immunohistochemical technique. RESULTS: All the specimens contained epithelial-like proliferative cells with or without pigment and some membrane-like pieces. Immunocytochemical staining showed that 76.0-90.0% cells stained for CK, 17.4-29.6% cells expressed GFAP, and 80.1-91.0% cells were positive for vimentin. CONCLUSIONS: Majority of cells in the vitreous samples originated from retinal pigment epithelial cells (RPE) and glial cells in PVR. Expression of IF proteins may be determined by tissue of origin and local microenvironment.
Authors:
B Liu; Y Hui; J Ma; H Du
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Yan ke xue bao = Eye science / "Yan ke xue bao" bian ji bu     Volume:  15     ISSN:  1000-4432     ISO Abbreviation:  Yan Ke Xue Bao     Publication Date:  1999 Mar 
Date Detail:
Created Date:  2003-02-12     Completed Date:  2004-07-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8605666     Medline TA:  Yan Ke Xue Bao     Country:  China    
Other Details:
Languages:  eng     Pagination:  13-6     Citation Subset:  IM    
Affiliation:
Department of Ophthalmology, Xijing Hospital, Fourth Military Medical University, Xian 710032, China.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Female
Glial Fibrillary Acidic Protein / analysis*
Humans
Immunohistochemistry
Intermediate Filaments / metabolism
Keratins / analysis
Male
Middle Aged
Neuroglia / metabolism
Pigment Epithelium of Eye / metabolism*
Vimentin / analysis
Vitreoretinopathy, Proliferative / metabolism*,  pathology
Vitreous Body / metabolism*
Chemical
Reg. No./Substance:
0/Glial Fibrillary Acidic Protein; 0/Vimentin; 68238-35-7/Keratins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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