Document Detail


Immunocytochemical localization of secretory acetylcholinesterase of the parasitic nematode Nippostrongylus brasiliensis.
MedLine Citation:
PMID:  7750137     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Various parasitic nematodes secrete acetylcholinesterase (AChE). In this study, the localization of AChE in the nematode Nippostrongylus brasiliensis and the secretory forms of AChE in culture fluid were examined. A thiocholine method revealed that AChE activity was localized in the subventral glands, which have a secretory and excretory function via a duct connected to the excretory pore. By electron microscopy, AChE activity was found mainly in the matrix of secretory granules, and sometimes in the Golgi apparatus in the subventral gland cells. These results show that nematode AChE is produced and stored in the subventral glands. Monoclonal antibodies against AChE of human erythrocytes or electric rays also bound to the nematode subventral gland, suggesting immuno-cross-reactivity of AChE among these species. When AChE activity in the nematode excretory-secretory product was examined by SDS polyacrylamide gel electrophoresis combined with the thiocholine method, intense activity was demonstrated as a single band at 74 kDa. Immunoblot analysis showed specific recognition of this molecule by IgE and IgG1 antibodies, but not by IgG2a antibody, in nematode-infected rat sera. These results indicate that the nematode AChE molecule produced in and secreted from the subventral glands is antigenic for the production of IgE/IgG1 in host animals.
Authors:
M Nakazawa; M Yamada; R Uchikawa; N Arizono
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell and tissue research     Volume:  280     ISSN:  0302-766X     ISO Abbreviation:  Cell Tissue Res.     Publication Date:  1995 Apr 
Date Detail:
Created Date:  1995-06-22     Completed Date:  1995-06-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0417625     Medline TA:  Cell Tissue Res     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  59-64     Citation Subset:  IM    
Affiliation:
Department of Medical Zoology, Kyoto Prefectural University of Medicine, Japan.
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MeSH Terms
Descriptor/Qualifier:
Acetylcholinesterase / analysis*,  immunology,  secretion
Animals
Antibodies, Helminth / immunology
Cytoplasmic Granules / enzymology
Golgi Apparatus / enzymology
Helminth Proteins / analysis*,  immunology,  secretion
Immunoglobulin E / immunology
Immunoglobulin G / classification,  immunology
Male
Nippostrongylus / anatomy & histology,  immunology,  metabolism*
Rats
Rats, Sprague-Dawley
Chemical
Reg. No./Substance:
0/Antibodies, Helminth; 0/Helminth Proteins; 0/Immunoglobulin G; 37341-29-0/Immunoglobulin E; EC 3.1.1.7/Acetylcholinesterase

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