Document Detail


Immunocytochemical localization of protein kinase-C subtypes in anterior pituitary cells: colocalization in hormone-containing cells reveals heterogeneity.
MedLine Citation:
PMID:  1935808     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have examined the distribution and colocalization of protein kinase-C (PKC) in cultured rat anterior pituitary cells by light microscopic immunocytochemistry using monoclonal antibodies to group A rat brain PKC subspecies. Type I (PKC gamma) was not detected in the cells, in line with the assertion that the gamma-enzyme is expressed specifically in central nervous tissues. The other subspecies recognized by the antibodies (PKC beta and PKC alpha) were present throughout the cytoplasm in a diffused pattern, while the nuclei were apparently unstained. The number of cells stained with the antibodies in juvenile animals (12 days old) increased rapidly with age and reached a plateau between adult (5-month-old) and older (1-yr-old) rats. Type II (PKC beta) was the predominant subspecies detected in anterior pituitary cells. Double immunofluorescence staining techniques enabled the colocalization of PKC with various anterior pituitary cell types. Surprisingly, not all of the hormone-producing cells were stained with the PKC antibodies. Moreover, within the different pituitary cell types, the percentage of PKC-stained cells varied, revealing heterogeneity among the various cell populations. Thus, among somatrophs, mammotrophs, thyrotrophs, ACTH-containing cells, and gonadotrophs, only 9%, 22%, 13%, 44%, and 26%, respectively, reacted with the PKC antibodies. We suggest that activation of pituitary PKC might mobilize only a fraction of the hormone-containing cells.
Authors:
S Garcia-Navarro; M Kalina; Z Naor
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Endocrinology     Volume:  129     ISSN:  0013-7227     ISO Abbreviation:  Endocrinology     Publication Date:  1991 Nov 
Date Detail:
Created Date:  1991-12-02     Completed Date:  1991-12-02     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0375040     Medline TA:  Endocrinology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2780-6     Citation Subset:  AIM; IM    
Affiliation:
Department of Cell Biology, University of Cordoba, Spain.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies / immunology
Antibody Specificity
Cell Aging
Cells, Cultured
Fluorescent Antibody Technique
Hormones / metabolism*
Immunohistochemistry
Pituitary Gland, Anterior / cytology,  metabolism*
Protein Kinase C / chemistry,  metabolism*
Tissue Distribution
Chemical
Reg. No./Substance:
0/Antibodies; 0/Hormones; EC 2.7.11.13/Protein Kinase C

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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