Document Detail

Immunocytochemical localization of endogenous anti-thrombin III in the vasculature of rat tissues reveals locations of anticoagulantly active heparan sulfate proteoglycans.
MedLine Citation:
PMID:  7930519     Owner:  NLM     Status:  MEDLINE    
We localized endogenous anti-thrombin III (ATIII) by light and electron microscopic immunocytochemical staining in cryostat and ultra-thin frozen sections of 10 different rat tissues, using rabbit alpha-human ATIII antibody that was shown to crossreact strongly with rat ATIII. EM immunocytochemical methods revealed discrete deposits of endogenous ATIII (absent after heparinase treatment), and thus by inference anticoagulantly active heparan sulfate proteoglycans (HSPGs) at a resolution of 10-20 nm, or an order of magnitude better than autoradiography or LM. ATIII was found in variable amounts almost entirely in the subendothelial space of blood vessels in various rat tissues. In kidney, ATIII was found immediately beneath the endothelium, in concentrated clusters associated with the vascular basement membrane. Equally important is the observed variation in expression of ATIII in the various tissues studied (i.e., kidney > liver, aorta, lung, spleen, adrenal > intestine, muscle, brain). On the basis of these observations, we confirm a model in which vascular abluminal and, perhaps to a much smaller extent, luminal anticoagulantly active HSPGs regulate coagulation mechanism activity, either by serving as a reserve of anticoagulant or by modulating the ambient function of the coagulation cascade.
Y Xu; H S Slayter
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society     Volume:  42     ISSN:  0022-1554     ISO Abbreviation:  J. Histochem. Cytochem.     Publication Date:  1994 Oct 
Date Detail:
Created Date:  1994-10-24     Completed Date:  1994-10-24     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9815334     Medline TA:  J Histochem Cytochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1365-76     Citation Subset:  IM    
Laboratory of Electron Microscopy, Dana Farber Cancer Institute, Boston, Massachusetts 02115.
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MeSH Terms
Antithrombin III / analysis*
Blood Coagulation
Blood Vessels / chemistry*,  ultrastructure
Fluorescent Antibody Technique
Heparan Sulfate Proteoglycans
Heparitin Sulfate / analysis*
Microscopy, Electron
Microscopy, Immunoelectron
Proteoglycans / analysis*
Rats, Sprague-Dawley
Grant Support
Reg. No./Substance:
0/Heparan Sulfate Proteoglycans; 0/Proteoglycans; 9000-94-6/Antithrombin III; 9050-30-0/Heparitin Sulfate

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