| Immunocytochemical localization of annexins V and VI in human placentae of different gestational ages. | |
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MedLine Citation:
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PMID: 8012449 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The cellular and subcellular localization of annexins V and VI, two members of a superfamily of Ca(2+)-dependent phospholipid- and membrane-binding proteins, was investigated in chorionic villi of human placentae of different gestational ages by postembedding immunocytochemistry at the electron microscope level. All cell types of placental villi, i.e., the syncytiotrophoblast, Langhans cells, Hofbauer cells, fibroblasts, and capillary endothelial cells, appeared to express the two proteins, irrespective of the gestational age. By immunogold particle counts, annexin V was observed to be 2-3 times as much abundant as annexin VI. Syncytiotrophoblast cells appeared to contain the largest amounts and Langhans cells appeared to contain the least amounts of annexins V and VI, as judged by immunocytochemistry. The two proteins were found associated with plasma, Golgi, and vacuolar membranes, and with membranes of the endoplasmic reticulum, as well as diffusely in the cytoplasm. Annexin V appeared to be distributed in nearly equal proportions between cell membranes and the cytoplasm in stromal cells and to be about 30% associated with cell membranes in trophoblast cells, whereas annexin VI appeared almost equally distributed between cell membranes and the cytoplasm in trophoblast and stromal cells. Also, annexins V and VI appeared to be more abundant in trophoblast cells than in stromal cells. The present data strongly support the idea that placenta is a preferential site of annexin-regulated activities, and suggest that annexins V and VI are actively involved in the Ca(2+)-dependent regulation of membrane processes in trophoblast cells. |
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Authors:
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M G Rambotti; A Spreca; R Donato |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cellular & molecular biology research Volume: 39 ISSN: 0968-8773 ISO Abbreviation: Cell. Mol. Biol. Res. Publication Date: 1993 |
Date Detail:
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Created Date: 1994-07-28 Completed Date: 1994-07-28 Revised Date: 2012-02-27 |
Medline Journal Info:
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Nlm Unique ID: 9316986 Medline TA: Cell Mol Biol Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 579-88 Citation Subset: IM |
Affiliation:
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Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Italy. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Annexin A5
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analysis* Annexin A6 / analysis* Calcium / metabolism Chorionic Villi / chemistry* Female Gestational Age Humans Immunohistochemistry Membrane Proteins / analysis Pregnancy Pregnancy Proteins / analysis* Subcellular Fractions / chemistry Trophoblasts / chemistry |
| Grant Support | |
ID/Acronym/Agency:
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27//Telethon |
| Chemical | |
Reg. No./Substance:
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0/Annexin A5; 0/Annexin A6; 0/Membrane Proteins; 0/Pregnancy Proteins; 7440-70-2/Calcium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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