Document Detail


Immunocytochemical identification of trichomonads.
MedLine Citation:
PMID:  6989339     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Trichomonas vaginalis is one of the more common pathogens of the genitourinary tract. Morphologic studies of trichomonal infections, however, have been hampered by the fragile nature of these thin-walled flagellates and the lack of specific histochemical procedures to identify the fragmented organisms found in tissue sections. These limitations have usually restricted positive identification to smears of freshly obtained specimens. In an attempt to overcome these limitations, a rabbit antiserum specific for Trichomonas organisms was produced by repeated subcutaneous injections of a documented strain of T vaginalis maintained in vitro. Using this antiserum, immunoperoxidase techniques have been modified so that trichomonads have been clearly identified both in smears and in paraffin-embedded tissue sections. This method seems to be useful in establishing the presence of these organisms in tissue and may subsequently contribute to the histopathologic definition of trichomoniasis in man.
Authors:
B D Bennett; J Bailey; W A Gardner
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Archives of pathology & laboratory medicine     Volume:  104     ISSN:  0003-9985     ISO Abbreviation:  Arch. Pathol. Lab. Med.     Publication Date:  1980 May 
Date Detail:
Created Date:  1980-06-27     Completed Date:  1980-06-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7607091     Medline TA:  Arch Pathol Lab Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  247-9     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Female
Humans
Immune Sera / isolation & purification
Immunoenzyme Techniques*
Male
Rabbits / immunology
Trichomonas Infections / microbiology
Trichomonas vaginalis / immunology*
Chemical
Reg. No./Substance:
0/Immune Sera

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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