Document Detail

Immunocytochemical identification of myoepithelial cells in normal and neoplastic rat mammary glands with the lectins Griffonia simplicifolia-1 and pokeweed mitogen.
MedLine Citation:
PMID:  2170504     Owner:  NLM     Status:  MEDLINE    
Peroxidase-conjugated Griffonia simplicifolia-1 (GS-1) and pokeweed mitogen (PWM) histochemically stain only the myoepithelial cells and not the epithelial or fibroblastic cells of rat mammary glands preserved in methacarn or glutaraldehyde and embedded in paraffin. This pattern of staining occurs in other rat exocrine glands except the pancreas, but is the reverse of that seen in most lining epithelium. The histochemical binding of GS-1 and PWM to myoepithelial cells is inhibited specifically by D-galactose and by polymers of N-acetylglucosamine, respectively. GS-1 and its subcomponent, GS-1-B4, also bind to extracellular structures similar to those stained by anti-laminin serum. At the ultrastructural level, both conjugated GS-1 and PWM bind to the plasma membrane of the myoepithelial cells, as well as to the adjacent basement membrane. Non-metastasizing rat mammary tumors produced by dimethylbenz[a]anthracene, by derivative epithelial stem-cell lines, and by a transplantable tumor all contain more elongated myoepithelium-like cells as well as cuboidal epithelium-like cells; both cell types are neoplastic. The more elongated myoepithelium-like cells are stained by GS-1 and PWM, whereas the cuboidal epithelium-like cells are unstained. Moderately and strongly metastatic rat mammary tumors produced by epithelial cell lines and by transplantable tumors, respectively, contain no such neoplastic cells that bind either lectin. We suggest that the carbohydrate receptors for GS-1 and PWM are consistent markers for the presence of the myoepithelial cell in normal and tumorous rat mammary glands.
C M Hughes; P S Rudland
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society     Volume:  38     ISSN:  0022-1554     ISO Abbreviation:  J. Histochem. Cytochem.     Publication Date:  1990 Nov 
Date Detail:
Created Date:  1990-11-16     Completed Date:  1990-11-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9815334     Medline TA:  J Histochem Cytochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1633-45     Citation Subset:  IM    
Department of Biochemistry, University of Liverpool, United Kingdom.
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MeSH Terms
Cells, Cultured
Epithelial Cells
Epithelium / metabolism
Immunohistochemistry / methods
Lectins / diagnostic use,  metabolism*
Mammary Glands, Animal / cytology*,  metabolism
Mammary Neoplasms, Experimental / metabolism,  pathology*
Mice, Inbred C57BL
Myoepithelioma / metabolism,  pathology
Plant Lectins*
Pokeweed Mitogens / diagnostic use,  metabolism*
Rats, Inbred WF
Tumor Cells, Cultured / metabolism,  pathology
Reg. No./Substance:
0/Griffonia simplicifolia lectins; 0/Lectins; 0/Plant Lectins; 0/Pokeweed Mitogens

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