Document Detail


Immunocytochemical detection of sulphonylated DNA in tissue sections. An alternative to Feulgen staining of DNA.
MedLine Citation:
PMID:  2482278     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We report here on a new sensitive and highly specific DNA staining technique which we have called sulpho-DNA staining. DNA staining is based on a sulphonylation reaction of 2'-deoxycytidine or cytidine that takes place in the 6th position of cytosine with ensuing immunodetection of the sulphonylated DNA. The specificity of DNA staining is introduced by the use of an antibody recognizing only modified DNA but not modified RNA, by recourse to an additional acid hydrolysis step which destroys RNA but not DNA. We describe here the optimal conditions for the sulphonylation of DNA using O-methylhydroxylamine and metabisulphite as reactants. The new DNA stain labels all nuclei in either normal human tissue or in tumor cells. For nuclear DNA the staining signal is higher for the sulpho-DNA staining than for the Feulgen staining for nuclear DNA. This new DNA staining technique is suitable for use on tissue sections as well as on cytosmears.
Authors:
P Fritz; B Oeffinger; H Multhaupt; A Mischlinski; C Klein; E Roller; H V Tuczek
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Histochemistry     Volume:  93     ISSN:  0301-5564     ISO Abbreviation:  Histochemistry     Publication Date:  1989  
Date Detail:
Created Date:  1990-03-14     Completed Date:  1990-03-14     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  0411300     Medline TA:  Histochemistry     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  175-81     Citation Subset:  IM    
Affiliation:
Robert Bosch Krankenhaus Stuttgart, Pathologisches Institut, Federal Republic of Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Monoclonal
Coloring Agents*
DNA / analysis*
DNA, Neoplasm / analysis*
Deoxycytidine
Humans
Hydroxylamines
Immunohistochemistry
Mice
Rosaniline Dyes*
Staining and Labeling / methods*
Sulfites
Sulfonic Acids / analysis*
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Coloring Agents; 0/DNA, Neoplasm; 0/Feulgen stain; 0/Hydroxylamines; 0/Rosaniline Dyes; 0/Sulfites; 0/Sulfonic Acids; 67-62-9/methoxyamine; 7681-57-4/sodium metabisulfite; 9007-49-2/DNA; 951-77-9/Deoxycytidine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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