Document Detail

Immunocytochemical detection of GABAergic nerve cells in the human temporal cortex using a direct gamma-aminobutyric acid antiserum.
MedLine Citation:
PMID:  3285956     Owner:  NLM     Status:  MEDLINE    
Recently, an immunocytochemical method using glutaraldehyde fixation and an antiserum developed against a GABA--glutaraldehyde--protein conjugate has permitted direct visualization of GABAergic structures in the brains of perfused animals. This paper reports a successful use of this technique on human temporal cortex fixed by immersion. The cerebral tissue was obtained from patients operated for focal epilepsy. GABA-positive somata, fibres and terminals are observed in all layers of the temporal cortex. Terminals are particularly abundant in the superficial portion of layer I and in layers II, III and IV. Dense plexuses of fibres are located in layers II, III, IV and VI and in the underlying white matter. Somata are found in all cortical layers and in the underlying white matter; they are round, oval, fusiform or triangular and exhibit a multipolar, bitufted or bipolar dendritic pattern. This technique for the visualization of GABAergic structures in the human brain may allow a better understanding of the pathogeny of epilepsy in which the GABAergic transmission has been implicated.
S Schiffmann; G Campistron; P Tugendhaft; J Brotchi; J Flament-Durand; M Geffard; J J Vanderhaeghen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Brain research     Volume:  442     ISSN:  0006-8993     ISO Abbreviation:  Brain Res.     Publication Date:  1988 Mar 
Date Detail:
Created Date:  1988-06-30     Completed Date:  1988-06-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0045503     Medline TA:  Brain Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  270-8     Citation Subset:  IM    
Laboratory of Neuropathology and Neuropeptide Research, Erasme Hospital, Brussels, Belgium.
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MeSH Terms
Immune Sera
Immunoenzyme Techniques
Neurons / cytology
Temporal Lobe / cytology*,  pathology
gamma-Aminobutyric Acid / analysis*,  immunology
Reg. No./Substance:
0/Immune Sera; 56-12-2/gamma-Aminobutyric Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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