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Immunocytochemical demonstration of vimentin in astrocytes and ependymal cells of developing and adult mouse nervous system.
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MedLine Citation:
PMID:  7026573     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The occurrence of vimentin, a specific intermediate filament protein, has been studied by immunoflourescence microscopy in tissue of adult and embryonic brain as well as in cell cultures from nervous tissue. By double imminofluorescence labeling, the distribution of vimentin has been compared with that of subunit proteins of other types of intermediate filaments (glial fibrillary acidic [GFA] protein, neurofilament protein, prekeratin) and other cell-type specific markers (fibronectin, tetanus toxin receptor, 04 antigen). In adult brain tissue, vimentin is found not only in fibroblasts and cells of larger blood vessels but also in ependymal cells and astrocytes. In embryonic brain tissue, vimentin is detectable as early as embryonic day 11, the earliest stage tested, and is located in radial fibers spanning the neural tube, in ventricular cells, and in blood vessels. At all stages tested, oligodendrocytes and neurons do not express detectable amounts of vimentin. In primary cultures of early postnatal mouse cerebellum, a coincident location of vimentin and GFA protein is seen in astrocytes, and both types of filament proteins are included in the perinuclear aggregates formed upon exposure of the cells to colcemid. In cerebellar cell cultures of embryonic-day-13 mice, vimentin is seen in various cell types of epithelioid or fibroblastlike morphology but is absent from cells expressing tetanus toxin receptors. Among these embryonic, vimentin-positive cells, a certain cell type reacting neither with tetanus toxin nor with antibodies to fibronectin or GFA protein has been tentatively identified as precursor to more mature astrocytes. The results show that, in the neuroectoderm, vimentin is a specific marker for astrocytes and ependymal cells. It is expressed in the mouse in astrocytes and glial precursors well before the onset of GFA protein expression and might therefore serve as an early marker of glial differentiation. Our results show that vimentin and GFA protein coexist in one cell type not only in primary cultures in vitro but also in the intact tissue in situ.
Authors:
J Schnitzer; W W Franke; M Schachner
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of cell biology     Volume:  90     ISSN:  0021-9525     ISO Abbreviation:  J. Cell Biol.     Publication Date:  1981 Aug 
Date Detail:
Created Date:  1981-12-15     Completed Date:  1981-12-15     Revised Date:  2010-06-22    
Medline Journal Info:
Nlm Unique ID:  0375356     Medline TA:  J Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  435-47     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Astrocytes / analysis*
Cells, Cultured
Central Nervous System / growth & development
Cerebellum / analysis
Ependyma / analysis*,  cytology
Fluorescent Antibody Technique
Glial Fibrillary Acidic Protein
Mice
Mice, Inbred Strains
Muscle Proteins / analysis*
Nerve Tissue Proteins / analysis
Spinal Cord / cytology
Vimentin
Chemical
Reg. No./Substance:
0/Glial Fibrillary Acidic Protein; 0/Muscle Proteins; 0/Nerve Tissue Proteins; 0/Vimentin
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): J Cell Biol
ISSN: 0021-9525
ISSN: 1540-8140
Publisher: The Rockefeller University Press
Article Information
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Print publication date: Day: 1 Month: 8 Year: 1981
Volume: 90 Issue: 2
First Page: 435 Last Page: 447
ID: 2111851
Publisher Id: 902435
PubMed Id: 7026573

Immunocytochemical demonstration of vimentin in astrocytes and ependymal cells of developing and adult mouse nervous system
J Schnitzer
WW Franke
M Schachner


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