Document Detail

Immunochemical studies on Rhodotorula gracilis D-amino acid oxidase.
MedLine Citation:
PMID:  1672654     Owner:  NLM     Status:  MEDLINE    
Polyclonal antibodies were prepared from rabbit sera after immunization with holo- and apo-D-amino acid oxidase purified from R. gracilis. Both anti-holo- and anti-apoenzyme IgG fractions (as well as affinity-purified IgG) were highly specific: in blot-transfer analyses after SDS-PAGE only a 39 kDa band, corresponding to enzyme monomer, was recognized even in the partially purified yeast extract. No cross-reaction was detected with pig kidney D-amino acid oxidase. As a difference from the mammalian enzyme, yeast D-amino acid oxidase anti-holo- and anti-apoenzyme IgGs had different properties in inactivation and precipitation experiments, indicating the existence of different antigenicity sites related to the FAD-binding domain in the enzyme.
L Pollegioni; M P Simonetta
Related Documents :
10780874 - A simple and efficient method for hemoglobin removal from mammalian tissue cytosol by z...
3801494 - The effects of reversible freezing inactivation and inhibitor binding on redox properti...
8615084 - Monoamine oxidase-b-positive granular structures in the hippocampus of aged senescence-...
8952084 - Evolution of eutherian cytochrome c oxidase subunit ii: heterogeneous rates of protein ...
8141854 - Eicosapentaenoic acid and docosahexaenoic acid suppress the proliferation of vascular s...
20795634 - Synthesis of symmetrical and unsymmetrical diarylalkynes from propiolic acid using pall...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experientia     Volume:  47     ISSN:  0014-4754     ISO Abbreviation:  Experientia     Publication Date:  1991 Mar 
Date Detail:
Created Date:  1991-05-03     Completed Date:  1991-05-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0376547     Medline TA:  Experientia     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  232-5     Citation Subset:  IM    
Department of General Physiology and Biochemistry, University of Milano, Italy.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Blotting, Western
D-Amino-Acid Oxidase / metabolism*
Electrophoresis, Polyacrylamide Gel
Precipitin Tests
Rhodotorula / enzymology*
Substrate Specificity
Reg. No./Substance:
EC Oxidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Characterization and detection of a newly described Asian taeniid using cloned ribosomal DNA fragmen...
Next Document:  Identification and possible biological relevance of spermatozoal transglutaminase.