Document Detail


Immunochemical pattern of aspartate aminotransferase isozumes in servral rodents and in Ehrlich ascites cells (38549).
MedLine Citation:
PMID:  47635     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Antisera against rat liver aspartate aminotransferase (EC 2.6.1.1) isozymes were used to study the activity and immunologic pattern of these isozymes in the livers of the rat, mouse, hamster, gerbil and in Ehrlich ascites cells. A double immunodiffusion precipitin test and immunoelectrophoresis showed that, except for the gerbil, there was a pattern of identity of AAT isozymes in the presence of either the antianionic or the anticationic antisera. Although gerbil AAT isozymes are immunochemically different from those of the other rodents studied, they were inactivated by the respective antiserum in a manner similar to that observed with the other species. This may suggest that antigenic determinants at the catalytic site of each of the liver aspartate aminotransferase isozymes are least likely to change throughout the evolutionary process.
Authors:
L Kopelovich
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)     Volume:  148     ISSN:  0037-9727     ISO Abbreviation:  Proc. Soc. Exp. Biol. Med.     Publication Date:  1975 Feb 
Date Detail:
Created Date:  1975-06-21     Completed Date:  1975-06-21     Revised Date:  2007-11-02    
Medline Journal Info:
Nlm Unique ID:  7505892     Medline TA:  Proc Soc Exp Biol Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  410-3     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Aspartate Aminotransferases / immunology,  metabolism*
Carcinoma, Ehrlich Tumor / enzymology*
Cricetinae
Cross Reactions
Epitopes
Gerbillinae
Immune Sera
Immunodiffusion
Immunoelectrophoresis
Isoenzymes / metabolism*
Liver / enzymology*
Male
Mice
Mice, Inbred C3H
Rats
Rats, Inbred BUF
Rodentia / metabolism*
Species Specificity
Chemical
Reg. No./Substance:
0/Epitopes; 0/Immune Sera; 0/Isoenzymes; EC 2.6.1.1/Aspartate Aminotransferases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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