Document Detail


Immunochemical detection of egg-white antigens and allergens in meat products.
MedLine Citation:
PMID:  10380777     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: The purpose of this study was to detect antigens and allergens in egg-white byproduct ingredients and after their incorporation in processed pork meat pastes. Commercially prepared foods may have potentially allergenic ingredients (egg, milk, soybean, wheat, and peanut) added in processing. Since allergic patients may react to unidentified ingredients, it is important to assess the allergenic potency of these food proteins added during processing. Egg white was chosen as an experimental model, since egg is one of the most prevalent allergens in food hypersensitivity. METHODS: Experimental pork meat pastes containing egg white underwent pasteurization and sterilization. Ingredients derived from egg-white or paste extracts were isoelectrofocused and then blotted onto cyanogen bromide-activated nitrocellulose membranes. Egg-white antigens were identified in ingredients and in meat products with rabbit anti-egg-white antiserum by isoelectric focusing immunoblotting. Allergens were identified with sera from sensitized patients. A sensitive ELISA test was developed to detect egg-white proteins in raw, pasteurized, and sterilized meat products. RESULTS: Antigens and allergens in four egg-white byproducts were detected. Egg-white antigens were detectable in all ingredients and meat pastes by ELISA. Allergens were detected in ingredients and in raw and pasteurized products by immunoprint techniques and ELISA. CONCLUSIONS: Masked egg-white allergens are recognized by human serum IgE after pasteurization. Egg-white antigens are detectable in sterilized meat by ELISA techniques. Ingestion of processed foods could entail a risk of allergic reactions for sensitized consumers.
Authors:
V Leduc; C Demeulemester; B Polack; C Guizard; L Le Guern; G Peltre
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Allergy     Volume:  54     ISSN:  0105-4538     ISO Abbreviation:  Allergy     Publication Date:  1999 May 
Date Detail:
Created Date:  1999-09-03     Completed Date:  1999-09-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7804028     Medline TA:  Allergy     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  464-72     Citation Subset:  IM    
Affiliation:
CTSCCV, Maisons-Alfort, France.
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MeSH Terms
Descriptor/Qualifier:
Allergens / analysis*
Animals
Antigens / analysis*
Egg Proteins / chemistry
Egg White / analysis*
Enzyme-Linked Immunosorbent Assay
Food Hypersensitivity*
Humans
Immunoblotting
Isoelectric Focusing
Meat Products / analysis*
Rabbits
Swine
Chemical
Reg. No./Substance:
0/Allergens; 0/Antigens; 0/Egg Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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