Document Detail


Immunochemical demonstration of alpha(s1)- and beta-casein in mouse mammary glands at early stages of pregnancy.
MedLine Citation:
PMID:  11799144     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We generated monoclonal antibodies (MAbs) against mouse alpha(s1)- and beta-casein and used them to survey casein immunochemically in mammary glands of mice at peri-coitous and pregnant stages. Two MAb-producing hybridoma cells, designated MCalpha1 cell and MCbeta1 cell, were established. Each antibody, when used in Western blotting, recognized specifically mouse alpha(s1)- and beta-casein among a wide spectrum of proteins of both a lactating mammary homogenate and mouse skim milk. Immunohistochemistry revealed alpha(s1)- and beta-casein in sections of lactating mammary glands. Staining was found in substances in the lumen and cytoplasm of duct and alveolar cells, particularly in rough endoplasmic reticulum and the Golgi apparatus. Mammary glands at Days 2, 4, 6, 8, and 14 of pregnancy showed positive staining specific to both alpha(s1)- and beta-casein in the lumen and cytoplasm of duct cells, whereas the glands at estrus and Day 0 of pregnancy were positive mainly for alpha(s1)-casein. Semiquantitative Western blotting analysis of both casein components in epithelial cell fractions from glands during pregnancy confirmed that intra-epithelial alpha(s1)- and beta-casein changed during three phases, elevated from trace levels to detectable levels during initial stages of pregnancy (Days 0, 2, and 4), declined to lower levels during mid-pregnancy (Days 6 and 8), and then rose to high levels during late pregnancy (Day 14).
Authors:
Takuya Kanazawa; Kaoru Kohmoto
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society     Volume:  50     ISSN:  0022-1554     ISO Abbreviation:  J. Histochem. Cytochem.     Publication Date:  2002 Feb 
Date Detail:
Created Date:  2002-01-18     Completed Date:  2002-03-19     Revised Date:  2004-11-09    
Medline Journal Info:
Nlm Unique ID:  9815334     Medline TA:  J Histochem Cytochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  257-64     Citation Subset:  IM    
Affiliation:
Department of Animal Breeding, Faculty of Agriculture, University of Tokyo, Tokyo, Japan. takuyak@msv.ipc.ibaraki.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Monoclonal
Blotting, Western
Caseins / chemistry,  immunology,  metabolism*
Epithelium / metabolism
Female
Immunohistochemistry
Lactation / metabolism
Mammary Glands, Animal / metabolism*
Mice
Mice, Nude
Pregnancy
Pregnancy, Animal / metabolism*
Rats
Rats, Sprague-Dawley
Time Factors
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Caseins
Comments/Corrections
Comment In:
J Histochem Cytochem. 2002 Jul;50(7):997-8   [PMID:  12094781 ]

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