| Immunochemical characteristics of a novel cell death receptor and a decoy receptor on granulosa cells of porcine ovarian follicles. | |
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MedLine Citation:
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PMID: 19002826 Owner: NLM Status: PubMed-not-MEDLINE |
Abstract/OtherAbstract:
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Previously, we prepared an IgM monoclonal antibody(PFG-1) which specifically recognized a cell-membraneglycoprotein (PFG-1 antigen; 55 kD, pI 5.9),immunohistochemically reacted with granulosa cells ofhealthy follicles but not of atretic follicles, andinduced granulosa cell apoptosis. In the presentstudy, an IgM monoclonal antibody (PFG-3) capable ofinducing granulosa cell apoptosis and an IgGmonoclonal antibody (PFG-4) not capable of inducingapoptosis were produced against granulosa cellsprepared from healthy antral follicles of porcineovaries. Two-dimensional Western blotting analysisrevealed that PFG-3 specifically recognized twocell-membrane proteins (named PFG-3-1 andPFG-3-2/PFG-1 antigens; 42 kD, pI 5.2 and 55 kD, pI5.9, respectively) of healthy granulosa cells, andthat PFG-4 recognized the same two cell-membraneproteins. In atretic granulosa cells, PFG-3-2/PFG-1antigen disappeared. Immunochemical reactions of theseantibodies were only detected in follicular granulosacells but not any other ovarian tissues or organs.PFG-3 and PFG-4 immunohistochemically reacted withgranulosa cells of healthy and atretic follicles. Whenthe isolated granulosa cells prepared from healthyfollicles were cultured in medium containing PFG-3,the cells underwent apoptosis, and co-incubation withPFG-4 inhibited PFG-3-inducible apoptosis. Theseobservations suggested that PFG-3-2/PFG-1 antigen isa novel cell death receptor which is different fromthe apoptosis-mediating receptors (Fas/Apo-1/CD95 orTNF receptor), and that PFG-3-1 antigen may act as adecoy receptor and inhibit apoptotic signal transmission. |
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Authors:
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N Manabe; A Myoumoto; C Tajima; M Fukumoto; M Nakayama; K Uchio; M Yamaguchi; H Miyamoto |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Cytotechnology Volume: 33 ISSN: 0920-9069 ISO Abbreviation: Cytotechnology Publication Date: 2000 Jul |
Date Detail:
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Created Date: 2008-11-12 Completed Date: 2011-07-14 Revised Date: 2013-03-27 |
Medline Journal Info:
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Nlm Unique ID: 8807027 Medline TA: Cytotechnology Country: Netherlands |
Other Details:
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Languages: eng Pagination: 189-201 Citation Subset: - |
Affiliation:
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Unit of Anatomy and Cell Biology, Department of Animal Sciences, Kyoto University, Kyoto, 606-8502, Japan, manabe@jkans.jkans.kais.kyoto-u.ac.jp. |
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