Document Detail


Immunochemical and biochemical analysis of the proliferating cell nuclear antigen (PCNA) in HeLa cells.
MedLine Citation:
PMID:  2869966     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PCNA, also known a cyclin, a protein of molecular weight (MW) 35 000, accumulates in the nuclei of dividing and transformed cells and reacts with autoantibodies from certain lupus patients. Using an indirect immunofluorescence technique, we show that lupus sera containing anti-PCNA antibodies reveal a heterogeneous nuclear fluorescence pattern upon reaction with asynchronous HeLa cells, whereas with synchronized cells a sequence of distinct patterns is disclosed on progression through the cell cycle. Cell-free translation of HeLa cell mRNA followed by immunoprecipitation with anti-PCNA sera shows a single protein with the same apparent MW as PCNA labelled in vivo, suggesting that PCNA is not derived from a larger precursor protein and not grossly modified by post-translational events. However, a group of at least nine nuclear polypeptides ranging in MW from about 12 000 to 110 000 are recognized by immunoblotting with anti-PCNA sera, indicating either that additional antigenic sites are produced on denaturation of native proteins or that additional autoantibodies are present in these sera. We also show that PCNA and several of these polypeptides are associated with nuclear structures containing chromatin.
Authors:
M R Sadaie; M B Mathews
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Experimental cell research     Volume:  163     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  1986 Apr 
Date Detail:
Created Date:  1986-05-01     Completed Date:  1986-05-01     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  423-33     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Antigens / analysis*
Autoantigens / analysis*
Cell Cycle
Hela Cells / cytology
Humans
Kinetics
Molecular Weight
Nucleoproteins / analysis*,  biosynthesis
Proliferating Cell Nuclear Antigen
Protein Biosynthesis
Grant Support
ID/Acronym/Agency:
CA 13106/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Antigens; 0/Autoantigens; 0/Nucleoproteins; 0/Proliferating Cell Nuclear Antigen

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