Document Detail


Immunochemical analysis of a monoclonal antibody recognizing a terminal glucuronic acid-containing epitope of insect acidic glycolipids.
MedLine Citation:
PMID:  1698706     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A cloned, hybridoma cell-line was established that secreted the monoclonal antibody CAF-I following stimulation of the donor Balb/c mouse spleen cells by the total acidic fraction glycolipids of the third-instar larvae of Calliphora vicina (Insecta:Diptera). The monoclonal antibody isotype was IgG3 By qualitative (TLC-immunostaining) and semi-quantitative (enzyme-linked immunosorbent assay) methods, and comparison with the cross-reactivity of known monoclonal antibodies, the epitope was specifically located on the terminal, non-reducing end of the oligosaccharide chain of most of the insect acidic glycolipids. Following isolation of the two main acidic glycolipids of C. vicina larvae (A5c and Az5c), exoglycosidase treatment characterized the terminal disaccharide CAF-I epitope as glucuronic acid bound to subterminal galactose, both in the beta-anomeric configuration: G1cA beta-Ga1 beta-. The immunohistological distribution of this epitope in the dipteran, Drosophila melanogaster, showed its main expression to be in the imaginal discs and brain of the third-instar larva, and the retinula cells of the ommatidial elements of the compound eye retina of the adult female.
Authors:
M Keller; R D Dennis; B Weske; H Wiegandt
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Hybridoma     Volume:  9     ISSN:  0272-457X     ISO Abbreviation:  Hybridoma     Publication Date:  1990 Aug 
Date Detail:
Created Date:  1990-11-15     Completed Date:  1990-11-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8202424     Medline TA:  Hybridoma     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  295-307     Citation Subset:  IM    
Affiliation:
Institut für Physiologische Chemie, Universität Marburg, FRG.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Monoclonal
Antibody Specificity
Carbohydrate Sequence
Chromatography, Thin Layer
Diptera / immunology*
Drosophila melanogaster / analysis
Enzyme-Linked Immunosorbent Assay
Epitopes / immunology
Female
Glycolipids / analysis*
Hybridomas
Immunohistochemistry
Larva / analysis
Molecular Sequence Data
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Epitopes; 0/Glycolipids

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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