| Immunoassay to quantify the major peach allergen Pru p 3 in foodstuffs. Differential allergen release and stability under physiological conditions. | |
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MedLine Citation:
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PMID: 12475298 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Pru p 3 is a lipid transfer protein (LTP) that has been identified as the major peach (Prunus persica) allergen. However, little is known about the amount present in both raw and processed foodstuffs. Moreover, the in vivo release upon consumption of peach-containing foods remains unclear. We have developed a sensitive monoclonal antibody-based ELISA for Pru p 3. The method has been applied to measure the allergen levels in foodstuffs and the allergen release under different physiological conditions. A significant variability in all raw peaches and peach-containing foods tested has been detected. The allergen was extracted more efficiently at a low pH, and it was highly resistant to pepsin. This ELISA will be very useful in controlling the allergen concentration in diagnostics, in evaluating threshold levels in provocation tests, and in detecting hidden allergens in processed foods and cosmetics. |
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Authors:
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Oscar A Duffort; Florentino Polo; Manuel Lombardero; Araceli Díaz-Perales; Rosa Sánchez-Monge; Gloria García-Casado; Gabriel Salcedo; Domingo Barber |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of agricultural and food chemistry Volume: 50 ISSN: 0021-8561 ISO Abbreviation: J. Agric. Food Chem. Publication Date: 2002 Dec |
Date Detail:
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Created Date: 2002-12-11 Completed Date: 2003-01-17 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0374755 Medline TA: J Agric Food Chem Country: United States |
Other Details:
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Languages: eng Pagination: 7738-41 Citation Subset: IM |
Affiliation:
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Departamento I+D, ALK-Abelló, SA, 28037 Madrid, Spain. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Allergens
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analysis*,
metabolism Antibodies, Monoclonal Drug Stability Enzyme-Linked Immunosorbent Assay* Food Preservation Fruit / immunology* Hydrogen-Ion Concentration Kinetics Pepsin A / metabolism Prunus / immunology* Reproducibility of Results Sensitivity and Specificity Solubility Solutions |
| Chemical | |
Reg. No./Substance:
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0/Allergens; 0/Antibodies, Monoclonal; 0/Pru p 3 allergen; 0/Solutions; EC 3.4.23.1/Pepsin A |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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