Document Detail


Immunoassay as an analytical tool in agricultural biotechnology.
MedLine Citation:
PMID:  16915826     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Immunoassays for biotechnology engineered proteins are used by AgBiotech companies at numerous points in product development and by feed and food suppliers for compliance and contractual purposes. Although AgBiotech companies use the technology during product development and seed production, other stakeholders from the food and feed supply chains, such as commodity, food, and feed companies, as well as third-party diagnostic testing companies, also rely on immunoassays for a number of purposes. The primary use of immunoassays is to verify the presence or absence of genetically modified (GM) material in a product or to quantify the amount of GM material present in a product. This article describes the fundamental elements of GM analysis using immunoassays and especially its application to the testing of grains. The 2 most commonly used formats are lateral flow devices (LFD) and plate-based enzyme-linked immunosorbent assays (ELISA). The main applications of both formats are discussed in general, and the benefits and drawbacks are discussed in detail. The document highlights the many areas to which attention must be paid in order to produce reliable test results. These include sample preparation, method validation, choice of appropriate reference materials, and biological and instrumental sources of error. The article also discusses issues related to the analysis of different matrixes and the effects they may have on the accuracy of the immunoassays.
Authors:
G David Grothaus; Murali Bandla; Thomas Currier; Randal Giroux; G Ronald Jenkins; Markus Lipp; Guomin Shan; James W Stave; Virginia Pantella
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review    
Journal Detail:
Title:  Journal of AOAC International     Volume:  89     ISSN:  1060-3271     ISO Abbreviation:  J AOAC Int     Publication Date:    2006 Jul-Aug
Date Detail:
Created Date:  2006-08-18     Completed Date:  2006-11-08     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9215446     Medline TA:  J AOAC Int     Country:  United States    
Other Details:
Languages:  eng     Pagination:  913-28     Citation Subset:  IM    
Affiliation:
EnviroLogix Inc, 500 Riverside Industrial Pkwy, Portland, ME 04103, USA. davidgrothaus@envirologix.com
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MeSH Terms
Descriptor/Qualifier:
Agriculture / methods*
Antibodies / chemistry
Antigens / chemistry
Biotechnology / methods*
Chemistry Techniques, Analytical / methods
Crops, Agricultural
Enzyme-Linked Immunosorbent Assay
Food Analysis / methods
Food, Genetically Modified
Genetic Engineering
Immunoassay / methods*
Plant Proteins / chemistry
Plants, Genetically Modified / immunology
Sensitivity and Specificity
Chemical
Reg. No./Substance:
0/Antibodies; 0/Antigens; 0/Plant Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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