| Immunoassay as an analytical tool in agricultural biotechnology. | |
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MedLine Citation:
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PMID: 16915826 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Immunoassays for biotechnology engineered proteins are used by AgBiotech companies at numerous points in product development and by feed and food suppliers for compliance and contractual purposes. Although AgBiotech companies use the technology during product development and seed production, other stakeholders from the food and feed supply chains, such as commodity, food, and feed companies, as well as third-party diagnostic testing companies, also rely on immunoassays for a number of purposes. The primary use of immunoassays is to verify the presence or absence of genetically modified (GM) material in a product or to quantify the amount of GM material present in a product. This article describes the fundamental elements of GM analysis using immunoassays and especially its application to the testing of grains. The 2 most commonly used formats are lateral flow devices (LFD) and plate-based enzyme-linked immunosorbent assays (ELISA). The main applications of both formats are discussed in general, and the benefits and drawbacks are discussed in detail. The document highlights the many areas to which attention must be paid in order to produce reliable test results. These include sample preparation, method validation, choice of appropriate reference materials, and biological and instrumental sources of error. The article also discusses issues related to the analysis of different matrixes and the effects they may have on the accuracy of the immunoassays. |
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Authors:
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G David Grothaus; Murali Bandla; Thomas Currier; Randal Giroux; G Ronald Jenkins; Markus Lipp; Guomin Shan; James W Stave; Virginia Pantella |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Review |
Journal Detail:
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Title: Journal of AOAC International Volume: 89 ISSN: 1060-3271 ISO Abbreviation: J AOAC Int Publication Date: 2006 Jul-Aug |
Date Detail:
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Created Date: 2006-08-18 Completed Date: 2006-11-08 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 9215446 Medline TA: J AOAC Int Country: United States |
Other Details:
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Languages: eng Pagination: 913-28 Citation Subset: IM |
Affiliation:
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EnviroLogix Inc, 500 Riverside Industrial Pkwy, Portland, ME 04103, USA. davidgrothaus@envirologix.com |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Agriculture
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methods* Antibodies / chemistry Antigens / chemistry Biotechnology / methods* Chemistry Techniques, Analytical / methods Crops, Agricultural Enzyme-Linked Immunosorbent Assay Food Analysis / methods Food, Genetically Modified Genetic Engineering Immunoassay / methods* Plant Proteins / chemistry Plants, Genetically Modified / immunology Sensitivity and Specificity |
| Chemical | |
Reg. No./Substance:
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0/Antibodies; 0/Antigens; 0/Plant Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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